Cystinyl aminopeptidase activity is decreased in renal cell carcinomas
Cystinyl aminopeptidase activity is decreased in renal cell carcinomas
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DOI：
10.1016/j.regpep.2007.06.002
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发表时间：
2007-12-04
影响因子：
--
通讯作者：
Varona, Adolfo
中科院分区：
文献类型：
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作者：
Larrinaga, Gorka;Lopez, Jose I.;Varona, Adolfo
近年来，人们对肽酶在几种肿瘤的癌变过程中的作用进行了研究。尽管肾脏是已知表达胱氨酸氨基肽酶（CAP）的主要组织之一，但对其在肾肿瘤中的作用知之甚少。本研究分析了三种主要肾癌：透明细胞癌（CCRCC）、乳头状肾细胞癌（PRCC）和憎色肾细胞癌（ChRCC）中膜结合和可溶性CAP的荧光活性。总的来说，与周围的非肿瘤组织相比，三种肾细胞癌的膜结合的CAP活性都明显降低。因此，肿瘤与非肿瘤的CAP比率（每毫克蛋白质的肽酶单位）如下：ccrcc为926 +/- 111比3778 +/- 276，prcc为737 +/- 181比4351 +/- 950，chrcc为592 +/- 118比4905 +/- 935。相比之下，在整个系列中，该酶的可溶性部分在比较肿瘤和非肿瘤CAP活性时表现出轻微和不显著的变化。经分期分级后，ccrcc表现出显著性差异：pT3类患者的膜结合活性水平明显高于pT1类患者，高分级患者（G3-4）的可溶性CAP活性高于低分级患者（G1-2）。这些数据可能为研究肾细胞癌患者的预后提供更多的可能性。(c) 2007 Elsevier B.V.版权所有
The involvement of peptidases in carcinogenetic processes of several tumor types has been researched in recent years. Although kidney is one of the major tissues known to express cystinyl-aminopeptidase (CAP), little is known about its role in renal neoplasia. This study analyzes fluorimetrically membrane-bound and soluble CAP activity in the three main renal cancers: clear cell (CCRCC), papillary (PRCC), and chromophobe (ChRCC) renal cell carcinomas. Overall, a marked decrease of membrane-bound CAP activity in all the three renal cell carcinomas was detected when compared with their respective surrounding non-tumor tissues. So, the tumor vs. non-tumor CAP ratios (units of peptidase per mg of protein) was as follows: 926 +/- 111 vs. 3778 +/- 276 for CCRCCs, 737 +/- 181 vs. 4351 +/- 950 for PRCCs, and 592 +/- 118 vs. 4905 +/- 935 for ChRCCs. In contrast, the soluble fraction of this enzyme displayed minor and non-significant changes when comparing tumor and non-tumor CAP activities in the whole series. After stratification by stage and grade, CCRCCs displayed significant differences: pT3 category had significantly higher levels of membrane-bound activity than pT1, and high grade cases (G3-4) had higher soluble CAP activity than low grade ones (G1-2). These data may open additional possibilities in the study of renal cell carcinoma with regard to the prognosis of patients. (c) 2007 Elsevier B.V. All rights reserved.