Double rolling circle replication (DRCR) is recombinogenic
Double rolling circle replication (DRCR) is recombinogenic
复制标题
DOI：
10.1111/j.1365-2443.2011.01507.x
复制
发表时间：
2011-05-01
期刊：
影响因子：
2.1
通讯作者：
Horiuchi, Takashi
中科院分区：
文献类型：
--
作者：
Okamoto, Haruko;Watanabe, Taka-aki;Horiuchi, Takashi
同源重组在维持遗传多样性和基因组稳定性方面起着至关重要的作用。在自然界中可以找到暗示超重组的有趣例子。在叶绿体DNA （cpDNA）和单纯疱疹病毒1 （HSV-1）基因组中，DNA序列两侧的反向重复序列非常频繁地发生反转，提示超重组事件。然而，导致这些事件的机制尚不清楚。我们之前在基于双滚圈复制（DRCR）的放大系统中观察到非常频繁的反转。在这里，利用酵母2 μ m质粒和扩增系统，我们发现DRCR与超重组事件密切相关。插入到这些系统中的反向重复或直接重复经常分别以drcr依赖的方式引起反转或删除/重复。基于这些观察结果，我们认为DRCR也可能参与与基因扩增和cpDNA和HSV基因组复制相关的自然发生的染色体重排。我们提出了一个DRCR显著刺激同源重组的模型。
Homologous recombination plays a critical role in maintaining genetic diversity as well as genome stability. Interesting examples implying hyper-recombination are found in nature. In chloroplast DNA (cpDNA) and the herpes simplex virus 1 (HSV-1) genome, DNA sequences flanked by inverted repeats undergo inversion very frequently, suggesting hyper-recombinational events. However, mechanisms responsible for these events remain unknown. We previously observed very frequent inversion in a designed amplification system based on double rolling circle replication (DRCR). Here, utilizing the yeast 2-mu m plasmid and an amplification system, we show that DRCR is closely related to hyper-recombinational events. Inverted repeats or direct repeats inserted into these systems frequently caused inversion or deletion/duplication, respectively, in a DRCR-dependent manner. Based on these observations, we suggest that DRCR might be also involved in naturally occurring chromosome rearrangement associated with gene amplification and the replication of cpDNA and HSV genomes. We propose a model in which DRCR markedly stimulates homologous recombination.