Characterization of the interaction of FTO protein with thioglycolic acid capped CdTe quantum dots and its analytical application
Characterization of the interaction of FTO protein with thioglycolic acid capped CdTe quantum dots and its analytical application
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FTO蛋白与巯基乙酸封端的CdTe量子点的相互作用表征及其分析应用
DOI：
10.1016/j.saa.2015.04.106
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发表时间：
2015-10-05
影响因子：
4.4
通讯作者：
Chang, Junbiao
中科院分区：
文献类型：
--
作者：
Ge, Baoyu;Li, Zhigang;Chang, Junbiao
以巯基乙酸（TGA）为稳定剂，在水溶液中合成了CdTe量子点。利用荧光、紫外-可见吸收、同步荧光和三维荧光光谱研究了TGA-CdTe量子点与脂肪质量和肥胖相关蛋白（FTO）的相互作用。结果表明，TGA-CdTe量子点可以通过静态猝灭过程强烈猝灭FTO蛋白的固有荧光。范德华键和氢键在稳定络合物中都起着重要作用。得到了不同温度下的结合常数和热力学参数。此外，我们发现添加FTO蛋白显著增强了量子点的荧光强度。在此基础上，建立了一种在5.52 × 10(-9) ~ 6.62 × 10(-7) mol L-1线性范围内检测Fro蛋白的灵敏方法，检出限为1.14 × 10(-9) mol L-1。研究了影响FTO蛋白与TGA-CdTe量子点相互作用的因素。(C) 2015 Elsevier B.V.版权所有
CdTe quantum dots (QDs) were synthesized in aqueous solution using thioglycolic acid (TGA) as stabilizing agents. The interaction between TGA-CdTe QDs and fat mass and obesity-associated (FTO) protein was investigated by fluorescence, UV-visible absorption, synchronous fluorescence and three-dimensional fluorescence spectroscopy. Results revealed that TGA-CdTe QDs could strongly quench the intrinsic fluorescence of FTO protein with a static quenching procedure. Both the van der Waals and hydrogen bonding played a major role in stabilizing the complex. The binding constant and thermodynamic parameters at different temperatures were obtained. In addition, we found that the fluorescence intensity of QDs was significantly enhanced by the addition of FTO protein. Based on this, a sensitive method for detecting Fro protein was obtained in the linear range of 5.52 x 10(-9)-6.62 x 10(-7) mol L-1 with the detection limit of 1.14 x 10(-9) mol L-1. The influences of factors on the interaction between FTO protein and TGA-CdTe QDs were studied. (C) 2015 Elsevier B.V. All rights reserved.