Chondroitin sulfates in developing mouse tooth germs. An immunohistochemical study with monoclonal antibodies against chondroitin-4 and chondroitin-6 sulfates.
Chondroitin sulfates in developing mouse tooth germs. An immunohistochemical study with monoclonal antibodies against chondroitin-4 and chondroitin-6 sulfates.
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小鼠牙胚发育过程中的硫酸软骨素。
DOI：
10.1111/j.1432-0436.1990.tb00428.x
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Ruch,JV
中科院分区：
文献类型：
--
作者：
Mark,MP;Baker,JR;Morrison,K;Ruch,JV
糖胺聚糖和蛋白聚糖在个体发育中的作用尚不清楚。发育中的牙齿为结构-功能关系的研究提供了一个潜在的重要模型。在这项研究中，我们分析了时间和空间的表达软骨素的不同硫酸化模式在胚胎磨牙和切牙。为此，我们使用了对未硫酸化、4-硫酸化和6-硫酸化形式的软骨素具有特异性的单克隆抗体（Mab），并结合间接免疫荧光或免疫过氧化物酶标记。未硫酸软骨素在胚胎牙齿中未检测到。4-硫酸软骨素和6-硫酸软骨素存在于星状网中，但除此之外，它们仅限于牙间充质。3B 3和MC 21 C-表位是6-硫酸软骨素的标志物，在芽期均匀分布在牙间充质中;随着发育的进行，它们从牙尖的牙乳头和切牙的前部区域消失。这些表位不存在于基底膜和前牙本质中。在成牙本质细胞谱系中，3B 3和MC 21 C-表位仅在分化早期的前成牙本质细胞之间检测到。单克隆抗体2B 6作为探针定位4-硫酸软骨素。这种糖胺聚糖早在牙板期就被检测到，但其表达仅限于牙齿的基底膜，直到钟状期晚期。牙尖形成开始后，在牙乳头的咬合区域也观察到强染色，而牙乳头的颈部区域保持256阴性。钟状期切牙2B 6免疫组化染色从前牙区到后牙区呈递减趋势。前牙本质细胞周围的细胞外基质与2B 6反应，由成牙本质细胞产生的前牙本质也被该抗体强烈标记。比较3B 3和2B 6在连续切片上的免疫染色，发现在牙发育过程中相应表位的分布模式是相互排斥的。此外，在连续生长的切牙中，发现3B 3和MC 21 C产生的免疫染色模式与牙齿前后轴沿着的有丝分裂指数之间存在显著的正相关。因此，硫酸软骨素似乎发育调节。我们推测，软骨素硫酸化模式的变化可能通过局部改变细胞外基质的功能特性在个体发育中发挥作用。
The role of glycosaminoglycans and proteoglyans during ontogenesis is not known. The developing tooth offers a potentially important model for studies of structure‐function relationships. In this study, we have analysed the temporal and spatial expression of chondroitins of differing sulfation patterns in embryonic molars and incisors. For this purpose, we have used monoclonal antibodies (Mabs) specific for unsulfated, 4‐sulfated, and 6‐sulfated forms of chondroitin in conjunction with indirect immuno‐fluorescence or immunoperoxidase labeling. Unsulfated chondroitin was not detected in embryonic teeth. Chondroitin 4‐ and chondroitin 6‐sulfates were present in the stellate reticulum but otherwise they were confined to the dental mesenchyme. The3B3andMC21C‐epitope, which are markers of 6‐sulfated chondroitin, were uniformly distributed in the dental mesenchyme during the bud stage; they disappeared from the dental papilla of the cusps and of the anterior region of the incisor as development proceeded. These epitopes were absent from the basement membrane and from the predentin. In the odontoblastic cell lineage, the3B3andMC21C‐epitopes were detected only between preodontoblasts at an early stage of differentiation. The monoclonal antibody2B6served as a probe to localize chondroitin 4‐sulfate. This glycosaminoglycan was detected as early as the dental lamina stage but its expression was restricted to the basement membrane of the teeth until the late bell stage. After the onset of cusp formation, strong staining was also observed over the occlusal region of the dental papilla while the cervical region of the dental papilla remained 256‐negative. Incisors at the bell stage exhibited a decreasing gradient of immunostaining by2B6from their anterior region to their posterior end. The extracellular matrix surrounding preodontoblasts reacted with2B6and the predentin, produced by the odontoblasts, was also intensely labeled with this antibody. Comparison between immunostaining with3B3and2B6, on consecutive sections revealed a mutually exclusive pattern of distribution of the corresponding epitopes during odontogenesis. Furthermore, in the continuously growing incisor, a striking positive correlation was found between the immunostaining patterns produced by3B3andMC21Cand the mitotic indices along the anterior‐posterior axis of the tooth. Hence, sulfation of chondroitin seems developmentally regulated. We postulate that changes in the sulfation pattern of chondroitin might play a role in ontogenesis by locally altering the functional properties of the extracellular matrix.