Biodegradable Nanoge-integrated Hydrogels for Sustained Protein Delivery
Biodegradable Nanoge-integrated Hydrogels for Sustained Protein Delivery
复制标题
可生物降解的纳米集成水凝胶用于持续蛋白质输送
DOI：
10.1007/s13233-012-0054-x
复制
发表时间：
2012
影响因子：
2.4
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Asako Shimoda;et al.
以丙烯酰基修饰的胆固醇普鲁兰多糖（CHPOA）为结构单元，巯基多臂聚乙二醇（PEGSH）为交联剂，制备了多糖纳米凝胶交联水凝胶。根据环境因素如pH和血清的存在，通过纳米凝胶和交联剂之间的酯键的裂解来释放固定化的纳米凝胶。将异硫氰酸荧光素标记的胰岛素（FITC-Ins）纳米凝胶与聚乙二醇（PEGSH）交联，制备了FITC-Ins水凝胶。蛋白质复合的纳米凝胶通过水解逐渐从水凝胶中释放出来，然后在牛血清白蛋白的存在下通过交换反应释放纳米凝胶中的蛋白质。CHPOA-PEGSH水凝胶可用作持续的蛋白质递送系统。
Polysaccharide nanogel-crosslinked hydrogels for sustained protein delivery systems were prepared using acryloyl group-modified cholesterol-bearing pullulan (CHPOA) as a building block and multiarmed poly(ethylene glycol) (PEGSH) with thiol groups as a cross-linker. Immobilized nanogels were released by the cleavage of ester bonds between the nanogels and cross-linkers according to environmental factors such as pH and the presence of serum. Fluorescein-isothiocyanate-labeled insulin (FITC-Ins)-containing hydrogel was prepared by the cross-linking of FITC-Ins-complexed nanogel and PEGSH. The protein-complexed nanogels were gradually released from the hydrogel by hydrolysis, and then the proteins in the nanogel were released by exchange reactions in the presence of bovine serum albumin. The CHPOA-PEGSH hydrogel is useful as a sustained protein delivery system.