Protocols for Assessing Transformation Rates of Nitrous Oxide in the Water Column
Protocols for Assessing Transformation Rates of Nitrous Oxide in the Water Column
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DOI：
10.3389/fmars.2021.611937
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发表时间：
2021-04-22
影响因子：
3.7
通讯作者：
Ward, Bess B.
中科院分区：
文献类型：
--
作者：
Bourbonnais, Annie;Frey, Claudia;Ward, Bess B.
一氧化二氮（N2O）是一种强效的温室气体和破坏臭氧的物质。然而，目前仍缺乏测量淡水和海洋环境中N2O转化率的明确的分步方案，这对相互比较的努力构成了挑战。在这里，我们介绍了该领域领先专家目前用于测量海洋和其他水生环境中水柱N2O产生和消耗率的详细协议。我们提出了在海洋环境中进行15n示踪剂孵育实验的例子，以及计算N2O产出率和消耗率的模板。我们讨论了以下方面的重要考虑和建议：(1)在低氧和缺氧培养过程中防止氧气（O2）污染的预防措施，(2)首选的瓶子和瓶塞，(3)添加15n示踪剂的程序，以及(4)固定剂的选择。我们最后讨论了数据报告和存档。我们期望这些协议将使15n标记的N2O转化率测量更容易被更广泛的社区获得，并促进未来不同实验室之间的相互比较。
Nitrous oxide (N2O) is a potent greenhouse gas and an ozone destroying substance. Yet, clear step-by-step protocols to measure N2O transformation rates in freshwater and marine environments are still lacking, challenging inter-comparability efforts. Here we present detailed protocols currently used by leading experts in the field to measure water-column N2O production and consumption rates in both marine and other aquatic environments. We present example 15N-tracer incubation experiments in marine environments as well as templates to calculate both N2O production and consumption rates. We discuss important considerations and recommendations regarding (1) precautions to prevent oxygen (O2) contamination during low-oxygen and anoxic incubations, (2) preferred bottles and stoppers, (3) procedures for 15Ntracer addition, and (4) the choice of a fixative. We finally discuss data reporting and archiving. We expect these protocols will make 15N-labeled N2O transformation rate measurements more accessible to the wider community and facilitate future inter-comparison between different laboratories.