A mutant tRNA affects delta-mediated transcription in Saccharomyces cerevisiae.
A mutant tRNA affects delta-mediated transcription in Saccharomyces cerevisiae.
复制标题
突变 tRNA 影响酿酒酵母中 δ 介导的转录。
DOI：
10.1093/genetics/132.2.361
复制
发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Winston,F
中科院分区：
文献类型：
--
作者：
Happel,AM;Winston,F
SPT 3、SPT 7、SPT 8和SPT 15基因中的突变定义了一类反式作用突变，其是由Ty元件或由Ty长末端重复序列δ引起的插入突变的强抑制因子。这些SPT基因是Ty元件正常转录所需的，并且它们的基因产物被认为参与了从δ序列开始Ty转录。我们分离并分析了spt 3突变的基因外抑制因子。这些新的突变称为rsp，部分抑制了对SPT 3、SPT 7、SPT 8和SPT 15功能的需求。此外，rsp突变会导致SPT+遗传背景中一些delta插入的转录发生变化。在四个确定的RSP基因突变之间的相互作用显示了一些有趣的遗传特性，包括不连锁的RSP突变，以补充隐性表型的失败。对其中一个rsp突变体基因rsp 4 -27的克隆和序列分析表明，它编码一个移码抑制甘氨酸tRNA。我们的研究结果表明，其他三个RSP基因也编码移码抑制甘氨酸tRNA。此外，其他类型的移码抑制甘氨酸tRNA可以赋予一些Rsp-表型。
Mutations in the SPT3, SPT7, SPT8 and SPT15 genes define one class of trans-acting mutations that are strong suppressors of insertion mutations caused by Ty elements or by the Ty long terminal repeat sequence, delta. These SPT genes are required for normal transcription of Ty elements, and their gene products are believed to be involved in initiation of Ty transcription from delta sequences. We have isolated and analyzed extragenic suppressors of spt3 mutations. These new mutations, named rsp, partially suppress the requirement for SPT3, SPT7, SPT8 and SPT15 functions. In addition, rsp mutations cause changes in transcription of some delta insertions in an SPT+ genetic background. Interactions between mutations in the four identified RSP genes show a number of interesting genetic properties, including the failure of unlinked rsp mutations to complement for recessive phenotypes. Cloning and sequencing of one rsp mutant gene, rsp4-27, showed that it encodes a frameshift suppressor glycine tRNA. Our results indicate that the other three RSP genes also encode frameshift suppressor glycine tRNAs. In addition, other types of frameshift suppressor glycine tRNAs can confer some Rsp- phenotypes.