Ventilatory effects of gap junction blockade in the NTS in awake rats
Ventilatory effects of gap junction blockade in the NTS in awake rats
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DOI：
10.1016/j.resp.2004.06.014
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发表时间：
2004-09-15
影响因子：
2.3
通讯作者：
Erlichman, JS
中科院分区：
文献类型：
--
作者：
Parisian, K;Wages, P;Erlichman, JS
我们测试的假设，局灶性灌注甘珀酸，阻断缝隙连接，到孤束核（NTS）将减少对CO2的兴奋反应。本研究测定了清醒状态下大鼠对吸入浓度递增的CO_2（FICO_2 = 0-8%）的每分钟通气量（V-E）、潮气量（V-T）和呼吸频率（F-R）的反应。乙酰唑胺（10 μ M）局部灌注到NTS增加V-E和V-T在暴露于室内空气。甘珀酸（300 μ M）降低和V-T反应CO2灌注时，但不邻近NTS小于10周龄的动物。在这些动物中，F-R在FICO 2 = 4%时降低。甘珀酸并没有减少V-E，V-T或F-R在动物10周龄及以上。甘珀酸不减少V-E，V-T或F-R时，局灶性灌注以外的NTS在任何年龄测试。NTS是所有年龄的重要CO2化学感受部位，间隙连接放大了小于10周龄动物对CO2的反应。(C)2004 Elsevier B. V.保留所有权利。
We tested the hypothesis that focally perfusing carbenoxolone, which blocks gap junctions, into the nucleus tractus solitatius (NTS) would reduce the ventilatory response to CO2. We measured minute ventilation (V-E), tidal volume (V-T) and respiratory frequency (F-R) responses to increasing concentrations of inspired CO2 (FICO2 = 0-8%) in rats during wakefulness. Focal perfusion of acetazolamide (10 muM) into the NTS increased V-E and V-T during exposure to room air. Carbenoxolone (300 muM) decreased the and V-T response to CO2 when perfused within, but not adjacent to the NTS in animals less than 10 weeks of age. F-R was decreased at FICO2 = 4% in these animals. Carbenoxolone did not decrease V-E, V-T or F-R in animals 10 weeks of age and older. Carbenoxolone did not decrease V-E, V-T or F-R when focally perfused outside the NTS at any age tested. The NTS is an important CO2 chemosensory site at all ages, and gap junctions amplify the ventilatory response to CO2 in animals less than 10 weeks of age. (C) 2004 Elsevier B.V. All rights reserved.