Topographic gene expression in the sinonasal cavity of patients with chronic sinusitis with polyps.
Topographic gene expression in the sinonasal cavity of patients with chronic sinusitis with polyps.
复制标题
与息肉的慢性鼻窦炎患者的鼻窦腔腔中的地形基因表达。
DOI：
10.1177/0194599811402030
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Stankovic KM
中科院分区：
文献类型：
--
作者：
Platt MP;Soler ZM;Kao SY;Metson R;Stankovic KM
To determine whether variations in gene expression exist at multiple subsites along the sinonasal tract in patients with chronic sinusitis with polyps and in healthy controls. Prospective, controlled study. Academic medical center. Tissue expression levels of 5 genes, previously found to be characteristic of ethmoid polyps, were measured using real-time quantitative polymerase chain reaction in 100 sinonasal tissue samples. Specimens harvested from 5 regions—the ethmoid sinus, septum, inferior turbinate, middle turbinate, and lateral nasal wall—in 10 patients with chronic sinusitis and ethmoid polyps were compared to tissue from similar regions in 10 control patients without sinusitis. Western blot analysis was performed to validate differential gene expression at the protein level. Gene expression levels of ethmoid polyps differed significantly from those of healthy ethmoid mucosa, as well as tissue from 4 surrounding anatomical sites in both patients with chronic sinusitis and controls. Alterations specific to the polyp tissue included downregulated genes, prolactin-induced protein (fold change 377.2 ± 169.0, P < .0001), and zinc α2-glycoprotein (fold change 72.1 ± 26.5, P < .0001), as well as upregulated genes, met proto-oncogene (fold change 2.5 ± 0.7, P = .029), and periostin (fold change 7.5 ± 3.4, P = .003). No significant differences in gene expression was found for neurabin 2 (fold change 1.0, P = .99). The transcriptional pattern of ethmoid polyps appears to be unique compared with other subsites in the sinonasal cavity of patients with chronic sinusitis. Care must be taken when collecting specimens for molecular studies of the sinonasal tract to differentiate polyp from nonpolyp tissue in chronic sinusitis.
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DOI：
10.1177/194589240602000205
发表时间：
2006-03-01
期刊：
AMERICAN JOURNAL OF RHINOLOGY
影响因子：
--
作者：
Shaari, J;Palmer, JN;Cohen, NA
通讯作者：
Cohen, NA
DOI：
10.1073/pnas.91.14.6259
发表时间：
1994-07-05
影响因子：
11.1
作者：
BAHRAM, S;BRESNAHAN, M;SPIES, T
通讯作者：
SPIES, T
影响因子：
3.4
作者：
Hao, J;Pang, YT;Wang, DY
通讯作者：
Wang, DY
影响因子：
14.2
作者：
Fritz, SB;Terrell, JE;Baker, JR
通讯作者：
Baker, JR
影响因子：
3.4
作者：
Gliklich, RE;Metson, R
通讯作者：
Metson, R