Targeted disruption of the mouse Asna1 gene results in embryonic lethality
Targeted disruption of the mouse Asna1 gene results in embryonic lethality
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DOI：
10.1016/j.febslet.2006.06.017
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发表时间：
2006-07-10
期刊：
影响因子：
3.5
通讯作者：
Bhattacharjee, Hiranmoy
中科院分区：
文献类型：
--
作者：
Mukhopadhyay, Rita;Ho, Ye-Shih;Bhattacharjee, Hiranmoy
细菌ArsA ATP酶是氧阴离子泵的催化组分，负责对砷和锑的抗性。细菌ArsA ATP酶的同源物在自然界中广泛存在。我们先前已经鉴定了与细菌ArsA ATP酶具有27%同一性的小鼠同源物（Asna 1）。为了鉴定蛋白质的生理作用，通过同源重组产生杂合Asna敲除小鼠（Asna 1（+/-））。Asna 1（+/-）小鼠表现出与野生型小鼠相似的表型。然而，在E3.5（E =胚胎日）和E8.5阶段之间，在纯合Asnal敲除胚胎中观察到早期胚胎致死。这些发现表明Asnal在早期胚胎发育中起着至关重要的作用。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The bacterial ArsA ATPase is the catalytic component of an oxyanion pump that is responsible for resistance to arsenicals and antimonials. Homologues of the bacterial ArsA ATPase are widespread in nature. We had earlier identified the mouse homologue (Asna1) that exhibits 27% identity to the bacterial ArsA ATPase. To identify the physiological role of the protein, heterozygous Asnal knockout mice (Asna1(+/-)) were generated by homologous recombination. The Asna1(+/-) mice displayed similar phenotype as the wild-type mice. However, early embryonic lethality was observed in homozygous Asnal knockout embryos, between E3.5 (E = embryonic day) and E8.5 stage. These findings indicate that Asnal plays a crucial role during early embryonic development. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.