Genomic organization of the sex-determining and adjacent regions of the sex chromosomes of medaka
Genomic organization of the sex-determining and adjacent regions of the sex chromosomes of medaka
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DOI：
10.1101/gr.5016106
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发表时间：
2006-07-01
期刊：
影响因子：
7
通讯作者：
Schartl, Manfred
中科院分区：
文献类型：
--
作者：
Kondo, Mariko;Hornung, Ute;Schartl, Manfred
人类Y染色体的测序揭示了旧进化时代异配子性染色体的基因组组织的特殊性，并导致了对其漫长历史中发生的进化变化的许多见解。我们已经研究了青鳉鱼Y染色体的基因组组织，这是最年轻的异配子性染色体上的分子数据。对Y特异性区域和邻近区域进行测序，并与X比较。男性性别决定基因，dmrt 1bY，似乎是唯一的功能基因在Y特异性区域。Y-特异性区域本身来自于来自连锁群9的43-kb片段的复制。除dmrt 1bY外，其余共复制基因均退化。Y-特异性区域积累了大量的重复序列和来自基因组其他地方的DNA复制片段，从而增长到258 kb。有趣的是，Y的非重组部分并没有从原始的重复片段中显著地扩散，这可能是因为与Y特异性片段接壤的大的序列重复。这可能保存了青鳉Y染色体的更古老的结构，并为Y染色体进化的一些初始过程提供了见解。
Sequencing of the human Y chromosome has uncovered the peculiarities of the genomic organization of a heterogametic sex chromosome of old evolutionary age, and has led to many insights into the evolutionary changes that occurred during its long history. We have studied the genomic organization of the medaka fish Y chromosome, which is one of the youngest heterogametic sex chromosomes on which molecular data are available. The Y specific and adjacent regions were sequenced and compared to the X. The male sex-determining gene, dmrt1bY, appears to be the only functional gene in the Y-specific region. The Y-specific region itself is derived from the duplication of a 43-kb fragment from linkage group 9. All other coduplicated genes except dmrt1bY degenerated. The Y-specific region has accumulated large stretches of repetitive sequences and duplicated pieces of DNA from elsewhere in the genome, thereby growing to 258 kb. Interestingly the non-recombining part of the Y did not spread out considerably from the original duplicated fragment, possibly because of a large sequence duplication bordering the Y-specific fragment. This may have conserved the more ancestral structure of the medaka Y and provides insights into some of the initial processes of Y chromosome evolution.