Longitudinal analysis on parasite diversity in honeybee colonies: new taxa, high frequency of mixed infections and seasonal patterns of variation
Longitudinal analysis on parasite diversity in honeybee colonies: new taxa, high frequency of mixed infections and seasonal patterns of variation
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DOI：
10.1038/s41598-020-67183-3
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发表时间：
2020-06-26
影响因子：
4.6
通讯作者：
Maside, Xulio
中科院分区：
文献类型：
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作者：
Bartolome, Carolina;Buendia-Abad, Maria;Maside, Xulio
为了评估寄生虫对意大利蜜蜂损失的影响，有必要监测它们随时间推移在蜂群中的存在。在这里，我们分析了发生的微孢子虫，锥虫和neoglitazines在五个同质的殖民地长达21个月，直到他们崩溃。这项研究结合了几种分子标记和大规模平行测序技术的应用，为这些寄生虫的流行病学提供了有价值的见解：（I）它能够检测蜜蜂中很少报告的寄生虫物种（微孢子虫、熊虫、棘头虫）的分离及两个新分类群的鉴定;（二）发现合并感染率高（80%的样品含有一种以上的寄生虫）;（III）发现了与新线虫不同的小孢子虫和锥虫的季节变化模式;（4）内蜂和外蜂的阳性样本比例和物种多样性水平均无显著差异;（5）寄生虫种类数的变化与蜂群失败无直接关系。
To evaluate the influence that parasites have on the losses of Apis mellifera it is essential to monitor their presence in the colonies over time. Here we analysed the occurrence of nosematids, trypanosomatids and neogregarines in five homogeneous colonies for up to 21 months until they collapsed. The study, which combined the use of several molecular markers with the application of a massive parallel sequencing technology, provided valuable insights into the epidemiology of these parasites: (I) it enabled the detection of parasite species rarely reported in honeybees (Nosema thomsoni, Crithidia bombi, Crithidia acanthocephali) and the identification of two novel taxa; (II) it revealed the existence of a high rate of co-infections (80% of the samples harboured more than one parasite species); (III) it uncovered an identical pattern of seasonal variation for nosematids and trypanosomatids, that was different from that of neogregarines; (IV) it showed that there were no significant differences in the fraction of positive samples, nor in the levels of species diversity, between interior and exterior bees; and (V) it unveiled that the variation in the number of parasite species was not directly linked with the failure of the colonies.