Growth of acid fast L forms from the blood of patients with sarcoidosis
Growth of acid fast L forms from the blood of patients with sarcoidosis
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DOI：
10.1136/thx.51.5.530
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发表时间：
1996-05-01
期刊：
影响因子：
10
通讯作者：
Mattman, LH
中科院分区：
文献类型：
--
作者：
Almenoff, PL;Johnson, A;Mattman, LH
背景-从结节病患者的血液、支气管洗涤液和眼前房液中培养出抗酸细胞壁缺陷型细菌。一种抗结核分枝杆菌全细胞抗原（H(37)RV）的单克隆抗体被用来表征结节病患者血液中CWDF的进一步生长。方法：采用有利于CWDF生长的方法培养20例活动性结节病患者和20例对照者的血液。分离株进一步通过间接荧光抗体分析进行特征分析，使用与结核分枝杆菌高度反应的单克隆抗体。结果：在20名结节病患者中，有19人的血液中培养出了CWDF。所有分离株单克隆抗体和改良Kinyoun染色均呈阳性。对照组的血液中没有生长出任何生物体。结论：这些数据表明CWDF可以从几乎所有活动性结节病患者的血液中生长。结果证实，这些微生物起源于分枝杆菌，与结核分枝杆菌相似，如果不是完全相同的话。它们在结节病发病机制中的作用尚不清楚。
Background - Acid fast cell wall deficient forms (CWDF) of bacteria have been grown from blood, bronchial washings, and ocular anterior chamber fluid from patients with sarcoidosis. A monoclonal antibody raised against Mycobacterium tuberculosis whole cell antigen (H(37)RV) was used to characterise further CWDF growth from the blood of patients with sarcoidosis.Methods - Blood from 20 patients with active sarcoidosis and from 20 controls was cultured using methods favourable for the growth of CWDF. Isolates were further characterised by indirect fluorescent antibody analysis using a monoclonal antibody highly reactive with M tuberculosis.Results - CWDF were grown from the blood of 19 of 20 subjects with sarcoidosis. All isolates stained positively with the monoclonal antibody and with a modified Kinyoun stain. No organisms were grown from the blood of controls.Conclusions - These data demonstrate that CWDF can be grown from the blood of nearly all patients with active sarcoidosis. The results confirm that the organisms are mycobacterial in origin and are similar, if not identical, to M tuberculosis. Their role in the pathogenesis of sarcoidosis is unknown.