[Expression and clinical significance of miR-23a and metastasis suppressor 1 in colon carcinoma].
[Expression and clinical significance of miR-23a and metastasis suppressor 1 in colon carcinoma].
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DOI：
10.3760/cma.j.issn.0529-5807.2012.01.008
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Hai-lin Tang;M. Deng;Q. Liao;Xi Zeng;Xiu-tian Zhou;Q. Su
中科院分区：
文献类型：
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作者：
Hai-lin Tang;M. Deng;Q. Liao;Xi Zeng;Xiu-tian Zhou;Q. Su
目的探讨miR-23 a和转移抑制因子1（MTSS 1）在结肠癌中的表达及其临床意义。方法收集92例结肠癌患者的癌组织和配对的正常组织标本。用荧光素酶报告载体检测靶向MTSS 1的miR-23 a。通过跨孔侵袭实验评估细胞侵袭能力。采用原位杂交和免疫组织化学方法检测miR-23 a和MTSS 1的表达。结果MiR-23 a下调MTSS蛋白的表达，增强结肠癌的侵袭性。miR-23 a和MTSS 1在结肠癌组织中的表达率分别为87.0%（80/92）和17.4%（16/92）（P < 0.01）。miR-23 a表达上调与临床分期（P = 0.029）和浸润深度（P = 0.000）相关。有淋巴结转移的胃癌组织中miR-23 a的表达高于无淋巴结转移的胃癌组织（P = 0.041）。MTSS 1表达下调与晚期临床分期（P = 0.027）和浸润深度（P = 0.017）相关。MTSS 1在有淋巴结转移组中的表达低于无淋巴结转移组（P = 0.009）。miR-23 a与MTSS 1的表达呈显著负相关（r =-0.594，P = 0.013）。结论miR-23 a的表达通过抑制MTSS基因的表达促进结肠癌细胞的生长、侵袭和转移。MTSS 1的低表达和miR-23 a的高表达可能是结肠癌恶性表型（如浸润和转移）的重要生物学标志。
OBJECTIVE To investigate the expression of miR-23a and metastasis suppressor 1 (MTSS1) and their clinical significance in colon carcinoma. METHODS A total of 92 cases of colon carcinomas were collected with both the tumor and paired normal tissue samples for the study. The miR-23a targeting MTSS1 was evaluated by luciferase reporter vector. Cell invasion potential was evaluated by trans-well invasion assay. In-situ hybridization and immunohistochemistry were used to detect miR-23a and MTSS1 expression. RESULTS MiR-23a downregulated the expression of MTSS protein and enhanced the invasiveness of colon carcinoma. The expression rates of miR-23a and MTSS1 were 87.0% (80/92) and 17.4% (16/92) in colon carcinoma cases, respectively (P < 0.01). The up-regulation of miR-23a expression was associated with an advanced clinical stage (P = 0.029) and depth of invasion (P = 0.000). The expression of miR-23a was higher in the tumors with lymph node metastasis than those without (P = 0.041). Down-regulation of MTSS1 expression was associated with an advanced clinical stage (P = 0.027) and depth of invasion (P = 0.017). The expression of MTSS1 was lower in the tumors with lymph node metastasis than those without (P = 0.009). The expression of miR-23a had significantly negative correlation with that of MTSS1 (r = -0.594, P = 0.013). CONCLUSIONS MiR-23a expression promotes colon carcinoma cell growth, invasion and metastasis through inhibition of MTSS gene. Both the low expression of MTSS1 and high expression of miR-23a may serve as important biological markers for the malignant phenotypes of colon cancer, such as invasion and metastasis.