Dissecting dynamin's role in clathrin-mediated endocytosis.
Dissecting dynamin's role in clathrin-mediated endocytosis.
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DOI：
10.1042/bst0371022
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发表时间：
2009-10
影响因子：
3.9
通讯作者：
Schmid SL
中科院分区：
文献类型：
--
作者：
Mettlen M;Pucadyil T;Ramachandran R;Schmid SL
GTPase Dynamin是细胞周期蛋白介导的细胞内吞作用的重要途径，但其确切的功能和作用机制一直存在争议。在这里，我们回顾了导致当前动力蛋白功能模型的发现，无论是作为机械力化学酶驱动膜分裂还是作为调节GTPase监控CME中的速率限制步骤。然而，这些模型并不是相互排斥的，随后的研究为这两种动力素功能提供了证据。在此，我们介绍了来自不同的体内和体外方法的最新证据，即Dynamin在CME中扮演双重角色，在早期作为保真度监测器调节笼状蛋白包裹的小孔成熟，在后期直接催化膜分裂和笼状蛋白包裹的囊泡的形成。
The GTPase dynamin is essential for clathrin-mediated endocytosis (CME), but its exact function and mechanism of action has been controversial. Here we review findings that have led to current models for dynamin function, either as a mechanochemical enzyme driving membrane fission or as a regulatory GTPase monitoring rate limiting steps in CME. However, these models are not mutually exclusive and subsequent studies have provided evidence for both dynamin functions. Here we present recent evidence derived from divergent in vivo and in vitro approaches that dynamin plays a dual role in CME, functioning at early stages as a fidelity monitor to regulate clathrin-coated pit maturation and at later stages to directly catalyze membrane fission and clathrin-coated vesicle formation.