Aberrant promoter methylation of p16INK4a and O6-methylguanine-DNA methyltransferase genes in workers at a Chinese uranium mine
Aberrant promoter methylation of p16INK4a and O6-methylguanine-DNA methyltransferase genes in workers at a Chinese uranium mine
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DOI：
10.1539/joh.48.261
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发表时间：
2006-07-01
影响因子：
3
通讯作者：
Tong, Jian
中科院分区：
文献类型：
--
作者：
Su, Shibiao;Jin, Yali;Tong, Jian
目的：探讨p16（INK 4a）基因甲基化与O-6-甲基鸟嘌呤-DNA甲基转移酶（O-6-Methylguanine-DNA Methyltransferase，O-6-Methylguanine-DNA Methyltransferase，O-6-Methylguanine-DNA Methyltransferase）的关系用O-6-MGMT法测定了某铀矿91名男性矿工的职业氡暴露量，按氡累积暴露剂量（2 ~ 425 WLM）分为4组（工作水平月），并通过特异性PCR检测确定痰液样本中p16（INK 4a）和O-6-MGMT基因的异常启动子甲基化。结果表明，随着氡累积剂量的增加，p16（INK 4a）基因（z=2.844，P=0.005）和O-6-MGMT基因（z=3.034，P=0.002）的甲基化率以及两个基因的总甲基化率（z=3.859，P=0.0001）均显著增加。这种甲基化可以作为一种潜在的标记，用于检测职业性氡暴露引起的早期DNA损伤。
To find the possible association of gene methylation of p16(INK4a) and O-6-Methylguanine-DNA Methyltransferase (O-6-MGMT) with occupational exposure to radon, 91 male miners from a uranium mine in China were divided into 4 groups according to the cumulative doses of radon exposure from 2 to 425 WLM (working-level months), and aberrant promoter methylation of p16(INK4a) and O-6-MGMT genes in sputum samples was determined by a specific PCR assay. The results revealed that the methylated rates of p16(INK4a) gene (z=2.844, P=0.005) and O-6-MGMT gene (z=3.034, P=0.002), and the total methylated rate of these two genes (z=3.859, P=0.0001) increased significantly with the cumulative doses of radon among the miners. This methylation could be applied as a potential marker for the detection of early DNA damage induced by occupational radon exposure.