Characterization of mitochondrial control region, two intergenic spacers and tRNAs of Zaprionus indianus (Diptera: Drosophilidae)
Characterization of mitochondrial control region, two intergenic spacers and tRNAs of Zaprionus indianus (Diptera: Drosophilidae)
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DOI：
10.1007/s10709-009-9396-5
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发表时间：
2009-12-01
期刊：
影响因子：
1.5
通讯作者：
Valiati, Victor Hugo
中科院分区：
文献类型：
--
作者：
da Silva, Norma Machado;Dias, Aline de Souza;Valiati, Victor Hugo
昆虫的控制区是动物线粒体DNA（mtDNA）中的主要非编码区，并且负责该细胞器的DNA序列和基因组大小的大部分变异。本研究克隆了一株印度扎普利翁（Zaprionus indianus）的mtDNA控制区、两个基因间隔区和tRNA基因，并对其进行了序列测定，同时与果蝇属其他物种进行了比较。Z. indianus控制区的保守性为94.3%，与其他果蝇物种的比较表明，最保守的区域似乎是距离tRNA最近的420个碱基对（ile），与其他作者的发现相似。我们还描述了保守的序列块，包括一个多聚T参与果蝇mtDNA的复制过程中，一个假定的二级结构也参与复制过程和重复序列。在物种间比较时，tRNA（ile）序列的变异性最大。
The control region in insects is the major noncoding region in animal mitochondrial DNA (mtDNA), and is responsible for a large part of the variation in the DNA sequence and size of the genome of this organelle. In this study, the mtDNA control region, two intergenic spacers and tRNA genes of a Zaprionus indianus strain were cloned, sequenced and compared with other Drosophila species. The overall A+T content in the Z. indianus control region is 94.3%, and a comparison with other Drosophila species demonstrated that the most conserved region appears to be the 420 base pairs nearest to the tRNA(ile), similar to the findings of other authors. We also describe conserved sequence blocks, including a poly-T involved in the replication process of Drosophila mtDNA; a putative secondary structure also involved in the replication process and repeated sequences. tRNA(ile) sequence demonstrated the greatest variability when the tRNA sequences of species were compared.