Elevated serum level of Thioredoxin in patients with Hepatocellular Carcinoma
Elevated serum level of Thioredoxin in patients with Hepatocellular Carcinoma
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DOI：
10.1023/a:1008032703468
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发表时间：
1998-01-01
期刊：
影响因子：
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通讯作者：
Wakasugi, H
中科院分区：
文献类型：
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作者：
Miyazaki, K;Noda, N;Wakasugi, H
硫氧还蛋白（TRX）是一种具有氧化还原活性的二硫化物，具有多种生物活性。本研究的目的是调查慢性肝炎（CH）或肝硬化（LC）和肝细胞癌（HCC）患者的循环TRX水平是否升高。产生特异性识别TRX的抗TRX单克隆抗体和多克隆抗体，并用于开发ELISA系统以测量人血清中的TRX水平。健康志愿者血清TRX水平的几何平均值及其95%可信区间为81.75 ng/ml（74.60-89.59 ng/ml），非肝癌LC/CH患者血清TRX水平为80.87 ng/ml（69.66-93.88 ng/ml）。该值与正常志愿者血清中的值无统计学差异（p=0.69）。肝癌患者血清TRX水平为147.35 ng/ml（125.53 ~ 172.96 ng/ml），明显高于正常人（P
Thioredoxin (TRX) is known to contain an active site with a redox-active disulfide and has various biological activities. The objective of the present study was to investigate whether circulating TRX levels are elevated in patients with chronic hepatitis (CH) or liver cirrhosis (LC) and hepatocellular carcinoma (HCC). An anti-TRX monoclonal antibody and polyclonal antibodies that specifically recognize TRX, were generated and used for the development of an ELISA system to measure TRX levels in human serum. The geometric mean and its 95% confidence interval of serum level of TRX in healthy volunteers was 81.75 ng/ml (74.60-89.59 ng/ml), The serum level of TRX in LC/CH patients without HCC was 80.87 ng/ml (69.66-93.88 ng/ml). The value was not statistically different from that in serum from normal volunteers (p=0.69). In contrast, the serum level of TRX in patients with HCC was 147.35 ng/ml (125.53-1 72.96 ng/ml), which was significantly higher when compared with the level in serum of normal volunteers (p