Prediction of multiple tandem OB-fold domains in telomere end-binding proteins Pot1 and Cdc13.
Prediction of multiple tandem OB-fold domains in telomere end-binding proteins Pot1 and Cdc13.
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端粒末端结合蛋白 Pot1 和 Cdc13 中多个串联 OB 折叠结构域的预测。
DOI：
10.1016/j.str.2004.07.015
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Wuttke,DeborahS
中科院分区：
文献类型：
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作者：
Theobald,DouglasL;Wuttke,DeborahS
heterodimericOxytricha novatelomere末端结合蛋白是一种原始的端粒末端结合蛋白，含有四个用于识别单链端粒DNA的OB-fold结构域。与此相反，只有孤立的OB-fold结构域已被发现在端粒末端结合蛋白从酵母和高等真核生物。使用滑动窗口算法加上序列轮廓轮廓分析，我们提供了支持的存在多个OB-折叠结构域在其他两个端粒的ssDNA结合蛋白，脊椎动物Pot 1和芽殖酵母Cdc 13。端粒末端结合蛋白中多个串联OB折叠结构域的这种常见用法扩展了真核生物末端保护机制的已知进化保守性。
The heterodimericOxytricha novatelomere end binding protein, the original telomere end binding protein characterized, contains four OB-fold domains used for recognition of single-stranded telomeric DNA. In contrast, only solitary OB-fold domains have been found in the telomere end binding proteins from yeast and higher eukaryotes. Using a sliding-window algorithm coupled with sequence profile-profile analysis, we provide support for the existence of multiple OB-fold domains in two other telomeric ssDNA binding proteins, vertebrate Pot1 and budding yeast Cdc13. This common usage of multiple, tandem OB-fold domains in telomeric end binding proteins extends the known evolutionary conservation of eukaryotic end-protection mechanisms.