Effects of hyperbaric oxygen preconditioning on ischemia‐reperfusion inflammation and skin flap survival
Effects of hyperbaric oxygen preconditioning on ischemia‐reperfusion inflammation and skin flap survival
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DOI：
10.3760/cma.j.issn.0366-6999.20121165
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发表时间：
2013-10
影响因子：
6.1
通讯作者：
Zheng Qi;Chun-jin Gao;You-Bin Wang;Xue-mei Ma;Ling Zhao;Fu-jia Liu;Xuehua Liu;Xue-jun Sun;Xiao-jun Wang
中科院分区：
文献类型：
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作者：
Zheng Qi;Chun-jin Gao;You-Bin Wang;Xue-mei Ma;Ling Zhao;Fu-jia Liu;Xuehua Liu;Xue-jun Sun;Xiao-jun Wang
背景高压氧预处理（HBO）是一种新的缺血预处理方法。在这项研究中，我们研究了其对皮瓣存活的影响及其机制。方法36只大鼠随机分为HBO预处理组、对照组和假手术组。以右腹壁浅动、静脉为蒂的扩大腹壁脂肪皮瓣。通过用微血管夹夹闭蒂血管诱导3小时的皮瓣缺血。缺血诱导结束后，取下钳夹，重新缝合皮瓣。高压氧预处理组大鼠在手术前接受4次高压氧治疗。于术后第1、3、5天测定皮瓣微循环。术后第5天处死动物前测量皮瓣大小。准备皮瓣样本，并用苏木精和伊红染色。测量皮瓣样本中肿瘤坏死因子（TNF）-、白细胞介素（IL）-1和IL-6的水平。结果高压氧预处理组皮瓣成活率明显高于对照组，炎症反应减轻，血流灌注增加。HBO预处理组的IL-1、TNF-α和IL-6水平低于对照组。结论高压氧预处理可提高缺血再灌注大鼠皮瓣的存活率。这种效应的机制可能与HBO预处理后炎症反应的减弱和皮瓣灌注增加有关。
Background Hyperbaric oxygen preconditioning (HBO) is a new method of ischemia preconditioning. In this study, we examined its effects on skin flap survival and the mechanisms involved. Methods Thirty‐six rats were divided into three groups: HBO preconditioning, control, and sham groups. An extended epigastric adipocutaneous flap based on the right superficial epigastric artery and vein was raised. A 3‐hour period of flap ischemia was induced by clamping the pedicle vessels with a microvascular clamp. At the end of ischemia induction, the clamp was removed and the flap was resutured. Rats in the HBO preconditioning group were treated with HBO four times before surgery. Microcirculation in the skin flap was measured on postoperative days 1, 3 and 5. The size of the flap was measured on postoperative day 5, before the animals were sacrificed. Samples of the skin flap were prepared and stained with hematoxylin and eosin. The levels of tumor necrosis factor (TNF)‐, interleukin (IL)‐1, and IL‐6 in the flap samples were measured. Results Surviving flap size was significantly higher in the HBO preconditioning group compared with controls, with a reduced inflammatory response and increased perfusion. IL‐1, TNF‐, and IL‐6 levels in the HBO preconditioning group were lower than in controls. Conclusions HBO preconditioning improved flap survival in this ischemia‐reperfusion rat model. The mechanisms responsible for this effect may relate to attenuation of the inflammatory response and increased flap perfusion following HBO preconditioning.