Type I collagen production by osteoblast-like cells cultured in contact with different bioactive glasses
Type I collagen production by osteoblast-like cells cultured in contact with different bioactive glasses
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DOI：
10.1002/jbm.a.10415
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发表时间：
2003-01-01
影响因子：
4.9
通讯作者：
Cannas, M
中科院分区：
文献类型：
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作者：
Bosetti, M;Zanardi, L;Cannas, M
生物活性玻璃是硅基的表面活性骨替代品，在骨和软组织中都表现出良好的生物相容性，用于口腔和颌面骨增强。先前的体外研究表明，生物活性玻璃支持大鼠成骨样细胞的生长和成熟，促进成骨表型的表达和维持，这表明对细胞活性有溶液介导和表面控制两种作用。在这项研究中，我们研究了三种不同生物活性玻璃接触培养的人原代成骨样细胞的行为，并将它们与以颗粒形式使用的无定形二氧化硅（SiO2）进行了比较。生物活性玻璃（45S5 Bioglass（(R)）含量为46.1% mol %）培养的碱性磷酸酶比活性在第2天和第4天显著高于对照培养和生物活性凝胶-玻璃（60 mol % （58S）和80 mol % (77S)）培养。成骨细胞合成I型胶原，随后矿化。免疫印迹和生化研究表明，与对照相比，与生物活性眼镜接触培养的成骨细胞样细胞释放的胶原蛋白增加。在三种生物活性玻璃中，45S5诱导成骨样细胞胶原蛋白释放量最高；此外，45S5处理后，I型胶原的mRNA被刺激了大约三到五倍。77S生物活性玻璃同样增加了I型胶原合成，即使碱性磷酸酶不高。这些结果表明，45S5生物玻璃（(R)）不仅能诱导人原代成骨样细胞的成骨分化，还能促进胶原的合成和释放。新配方的生物活性凝胶玻璃77S似乎在组织工程中有潜在的应用，诱导胶原合成增加。(C) 2002 Wiley期刊有限公司
Bioactive glasses are silica-based, surface-active bone substitutes that have shown good biocompatibility both in bone and in soft tissue and are used in oral and maxillofacial bone augmentation. Previous in vitro studies showing that bioactive glasses support the growth and maturation of rat osteoblast-like cells and promote the expression and maintenance of the osteoblastic phenotype have suggested that there is both a solution-mediated and a surface-controlled effect on cell activity. In this study, we investigated the behavior of human primary osteoblast-like cells cultured in contact with three different bioactive glasses and compared them with amorphous silica (SiO2) used in the form of granules. The specific activity of alkaline phosphatase determined biochemically was significantly higher at 2 and 4 days on the bioactive glass with 46.1 mol % silica content (45S5 Bioglass((R))) cultures than in the control cultures and in the bioactive gel-glass cultures, which had 60 mol % (58S) and 80 mol % (77S) silica content. Osteoblasts synthesize collagen type I, which is subsequently mineralized. Immunoblot and biochemical studies showed increased collagen release from osteoblast-like cells cultured in contact with bioactive glasses over that of controls. Among the three bioactive glasses, 45S5 is the highest inducer of osteoblast-like cell collagen release; moreover, mRNA for type I collagen was stimulated approximately three- to fivefold after 45S5 treatment. 77S bioactive glass similarly increased type I collagen synthesis even though alkaline phosphatase was not higher. These results suggest that 45S5 Bioglass((R)) not only induces osteogenic differentiation of human primary osteoblast-like cells, but can also increase collagen synthesis and release. The newly formulated bioactive gel-glass 77S seems to have potential applications for tissue engineering, inducing increased collagen synthesis. (C) 2002 Wiley Periodicals, Inc.