Membrane‐SPINE: An improved method to identify protein–protein interaction partners of membrane proteins in vivo
Membrane‐SPINE: An improved method to identify protein–protein interaction partners of membrane proteins in vivo
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DOI：
10.1002/pmic.201000558
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发表时间：
2011-05
期刊：
影响因子：
3.4
通讯作者：
V. Müller;P. Jungblut;T. Meyer;S. Hunke
中科院分区：
文献类型：
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作者：
V. Müller;P. Jungblut;T. Meyer;S. Hunke
膜蛋白对于许多基本的细胞过程是至关重要的。由于膜蛋白在复合物中起作用，因此无疑需要检测和表征膜蛋白-蛋白相互作用的方法。因此，我们开发了“膜-Strep-标记蛋白相互作用实验”（Membrane‐SPINE），该实验将Strep-标记膜蛋白的特异性纯化与通过甲醛交联可逆固定蛋白复合物相结合。结合MS分析，我们建议Membrane‐SPINE作为一种强大的工具来识别体内膜蛋白的未知相互作用伙伴。
Membrane proteins are crucial for many essential cellular processes. As membrane proteins function in complexes, methods to detect and to characterize membrane protein–protein interactions are undoubtedly required. Therefore, we developed the “Membrane‐Strep‐tagged protein interaction experiment” (Membrane‐SPINE) that combines the specific purification of a Strep‐tagged membrane protein with the reversible fixation of protein complexes by formaldehyde cross‐linking. In combination with MS analysis, we suggest Membrane‐SPINE as a powerful tool to identify unknown interaction partners of membrane proteins in vivo.