Ultramicroscopy: 3D reconstruction of large microscopical specimens
Ultramicroscopy: 3D reconstruction of large microscopical specimens
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DOI：
10.1002/jbio.200710011
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发表时间：
2008-02-01
影响因子：
2.8
通讯作者：
Dodt, H. -U
中科院分区：
文献类型：
--
作者：
Becker, K.;Jaehrling, N.;Dodt, H. -U
超微技术是一种可以对生物和医学标本进行光学切片和3D重建的显微技术。虽然在共聚焦显微镜下，样品大小最多只有几百微米，但使用超微显微镜，即使是厘米大小的物体，如整个小鼠胚胎，也可以以微米级的分辨率重建。这可以通过结合使用清除程序和光片照明原理来实现。我们提供了整个免疫组织化学标记的小鼠胚胎和成年果蝇的超微3D重建，详细了解了它们的解剖结构。它的快速和简单使超微技术非常适合于转基因小鼠的高通量表型筛选，从而有助于疾病模型的研究。(C)2008年，威利-VCH Verlag GmbH&Co.KGaA，Weinheim
Ultramicroscopy is a microscopical technique that allows optical sectioning and 3D reconstruction of biological and medical specimens. While in confocal microscopy specimen size is limited to several hundred micrometers at best, using ultramicroscopy even centimeter sized objects like whole mouse embryos can be reconstructed with micrometer resolution. This is possible by using a combination of a clearing procedure and the principle of lightsheet illumination. We present ultramicroscopic 3D reconstructions of whole immunohistochemically labelled mouse embryos and adult Drosophila, giving detailed insight into their anatomy. Its speed and simplicity makes ultramicroscopy ideally suited for high-throughput phenotype screening of transgenic mice and thus will benefit the investigation of disease models. (C) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim