MONOCLONAL-ANTIBODY K1 REACTS WITH EPITHELIAL MESOTHELIOMA BUT NOT WITH LUNG ADENOCARCINOMA
MONOCLONAL-ANTIBODY K1 REACTS WITH EPITHELIAL MESOTHELIOMA BUT NOT WITH LUNG ADENOCARCINOMA
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DOI：
10.1097/00000478-199203000-00006
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发表时间：
1992-03-01
影响因子：
5.6
通讯作者：
WILLINGHAM, MC
中科院分区：
文献类型：
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作者：
CHANG, K;PAI, LH;WILLINGHAM, MC
对人类肿瘤组织低温切片的免疫过氧化物酶组织化学染色显示，鼠单克隆抗体 (MAb) K1 可以区分上皮间皮瘤和肺腺癌。所有15个上皮型间皮瘤样本和所有4个混合型间皮瘤样本，但23个具有不同组织学分化程度的肺腺癌均未表现出与抗体K1的反应性。在每个测试的间皮瘤细胞群中，80% 至 100% 显示出强烈且均匀的 MAb K1 染色。对来自上皮样间皮瘤 (H-meso) 和几种肺癌细胞尖的活培养细胞以及间皮瘤患者的胸腔积液的免疫荧光分析也显示 K1 与间皮瘤细胞的选择性反应。这些数据表明，K1 可用作间皮细胞标记物，用于上皮型间皮瘤的鉴别病理诊断。 K1 还可用于上皮型和混合型人类恶性间皮瘤的发病机制、免疫诊断和免疫治疗的研究。
Immunoperoxidase histochemical staining of cryostat sections from human tumor tissues revealed that a murine monoclonal antibody (MAb), K1, can distinguish epithelia mesotheliomas from lung adenocarcinomas. All of 15 epithelial-type mesotheliomas and all four mixed type mesothelioma samples, but none of 23 lung adenocarcinomas with different degrees of histologic differentiation demonstrated reactivity with antibody K1. Of the cell populations in each mesothelioma tested, 80% to 100% showed strong and homogeneous staining with MAb K1. Immunofluorescence analysis of live cultured cells from an epithelioid mesothelioma (H-meso) and several lung carcinoma cell tines as well as a pleural effusion of a patient with mesothelioma also showed selective reactivity of K1 with the mesothelioma cells. These data indicate that K1 can be useful as a mesothelial cell marker for the differential pathological diagnosis of the epithelial form of mesothelioma; K1 may also be useful in the study of the pathogenesis, immunodiagnosis, and immunotherapy of epithelial-type and mixed-type human malignant mesothelioma.