Self-Assembled Hexagonal Superparamagnetic Cone Structures for Fabrication of Cell Cluster Arrays
Self-Assembled Hexagonal Superparamagnetic Cone Structures for Fabrication of Cell Cluster Arrays
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DOI：
10.1021/acsami.0c17890
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发表时间：
2021-03-01
影响因子：
9.5
通讯作者：
Liu, Xiaoxi
中科院分区：
文献类型：
--
作者：
Chen, Yinling;Hu, Zhixin;Liu, Xiaoxi
在这项研究中，我们表明，阵列的细胞团簇可以制造自组装的六方超顺磁性锥结构。当玻璃衬底上的铁磁流体受到强的面外磁场作用时，会在连续的铁磁流体上下表面上感应出磁极，从而增加静磁能。然后，铁磁流体将经历流体动力学不稳定性，并分裂成具有锥形结构的小液滴，因为表面张力能和静磁能的折衷使系统的总能量最小化。此外，铁磁流体锥有序地自组装成六边形阵列，达到最低能量状态。在这些液体锥脱水形成固体锥后，聚二甲基硅氧烷被浇铸以固定六方超顺磁性锥结构的排列并防止磁性纳米颗粒的泄漏。通过与铁磁流体共培养中的内吞作用，用磁性纳米颗粒标记U-343人神经元胶质母细胞瘤细胞。通过细胞磁电泳分析，内化的磁性纳米颗粒的数量为（4.2 +/-0.84）X 10（6）/细胞。这些磁性标记的细胞被六方超顺磁性锥结构吸引并捕获，形成细胞簇阵列。作为固体锥尺寸的函数，在2000 G的面外磁场下，由每个六边形超顺磁性锥结构捕获的细胞的数量从48增加到126。从细胞磁电泳得到的六方超顺磁锥的局部磁场梯度为117.0- 140.9G/mm。当施加外部磁场时，我们观察到细胞边缘的突起数量从荧光图像中减少。结果表明，超顺磁性六角锥体产生的局部磁场梯度限制了细胞的生长和迁移。
In this study, we demonstrated that arrays of cell clusters can be fabricated by self-assembled hexagonal superparamagnetic cone structures. When a strong out-of-plane magnetic field was applied to the ferrofluid on a glass substrate, it will induce the magnetic poles on the upper/lower surfaces of the continuous ferrofluid to increase the magnetostatic energy. The ferrofluid will then experience hydrodynamic instability and be split into small droplets with cone structures because of the compromising surface tension energy and magnetostatic energy to minimize the system's total energy. Furthermore, the ferrofluid cones were orderly self-assembled into hexagonal arrays to reach the lowest energy state. After dehydration of these liquid cones to form solid cones, polydimethylsiloxane was cast to fix the arrangement of hexagonal superparamagnetic cone structures and prevent the leakage of magnetic nanoparticles. The U-343 human neuronal glioblastoma cells were labeled with magnetic nanoparticles through endocytosis in co-culture with a ferrofluid. The number of magnetic nanoparticles internalized was (4.2 +/- 0.84) x 10(6) per cell by the cell magnetophoresis analysis. These magnetically labeled cells were attracted and captured by hexagonal superparamagnetic cone structures to form cell cluster arrays. As a function of the solid cone size, the number of cells captured by each hexagonal superparamagnetic cone structure was increased from 48 to 126 under a 2000 G out-of-plane magnetic field. The local magnetic field gradient of the hexagonal superparamagnetic cone was 117.0-140.9 G/mm from the cell magnetophoresis. When an external magnetic field was applied, we observed that the number of protrusions of the cell edge decreased from the fluorescence images. It showed that the local magnetic field gradient caused by the hexagonal superparamagnetic cones restricted the cell growth and migration.