Electrochemical Biosensor for DNA Methylation Detection through Hybridization Chain-Amplified Reaction Coupled with a Tetrahedral DNA Nanostructure
Electrochemical Biosensor for DNA Methylation Detection through Hybridization Chain-Amplified Reaction Coupled with a Tetrahedral DNA Nanostructure
复制标题
通过与四面体 DNA 纳米结构耦合的杂交链放大反应检测 DNA 甲基化的电化学生物传感器
DOI：
10.1021/acsami.8b20144
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发表时间：
2019-01-30
影响因子：
9.5
通讯作者：
Zheng, Junsong
中科院分区：
文献类型：
--
作者：
Chen, Xi;Huang, Jian;Zheng, Junsong
DNA甲基化是基因表达疾病或恶性肿瘤发病机制的关键因素。因此，它已成为这些疾病的诊断和预后的重要生物标志物。在本文中，我们设计了一种用于 DNA 甲基化检测的超灵敏且特异的电化学生物传感器。该平台由锚定在金纳米颗粒涂层金电极上的茎环四面体复合 DNA 探针、HpaII 的限制性酶消化以及包括金纳米颗粒电沉积、杂交链式反应和辣根过氧化物酶催化在内的信号放大程序组成。在最佳条件下，该设计显示出 1 aM 至 1 pM 的宽动态范围以及约 0.93 aM 的检测限。该方法还表现出理想的特异性、可重复性和稳定性。回收率测试表明，该设计是临床环境下DNA甲基化检测的一个有前景的平台，可以满足癌症诊断的需求。
DNA methylation is a key factor in the pathogenesis of gene expression diseases or malignancies. Thus, it has become a significant biomarker for the diagnosis and prognosis of these diseases. In this paper, we designed an ultrasensitive and specific electrochemical biosensor for DNA methylation detection. The platform consisted of stem-loop-tetrahedron composite DNA probes anchoring at a Au nanoparticle-coated gold electrode, a restriction enzyme digestion of HpaII, and signal amplification procedures including electrodeposition of Au nanoparticles, hybridization chain reaction, and horseradish peroxidase enzymatic catalysis. Under optimal conditions, the design showed a broad dynamic range from 1 aM to 1 pM and a detection limit of about 0.93 aM. The approach also showed ideal specificity, repeatability, and stability. The recovery test demonstrated that the design is a promising platform for DNA methylation detection under clinical circumstances and could meet the need for cancer diagnosis.