Investigation of space flight effects on Escherichia coli and a proposed model of underlying physical mechanisms
Investigation of space flight effects on Escherichia coli and a proposed model of underlying physical mechanisms
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DOI：
10.1099/00221287-143-2-449
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发表时间：
1997-02-01
期刊：
影响因子：
2.8
通讯作者：
Stodieck, L
中科院分区：
文献类型：
--
作者：
Klaus, D;Simske, S;Stodieck, L
以前的研究报告说，空间飞行可能对微生物代谢产生刺激作用;然而，尚未确定与所观察到的变化有关的具体潜在机制。为了系统地评价空间飞行对微生物生长各个阶段（滞后期、指数期和静止期）的影响，在美国航天飞机的七次飞行任务中使用大肠杆菌的体外悬浮培养物进行了一系列实验。结果表明，由于空间飞行，滞后期缩短，指数生长持续时间增加，最终细胞群体密度大约增加了一倍。从这些累积数据中推导出一个模型，试图将重力依赖性细胞外转运现象与在每个特定生长阶段观察到的独特变化联系起来。有人建议，重力的累积效应可能会有一个显着的影响，悬浮细胞通过其流体环境中，立即，直接的影响，否则重力可能被认为是微不足道的。
Previous investigations have reported that space flight may produce a stimulating effect on microbial metabolism; however, the specific underlying mechanisms associated with the observed changes have not yet been identified. In an effort to systematically evaluate the effect of space flight on each phase of microbial growth (lag, exponential and stationary), a series of experiments was carried out using in vitro suspension cultures of Escherichia coli aboard seven US Space Shuttle missions. The results indicated that, as a result of space flight, the lag phase was shortened, the duration of exponential growth was increased, and the final cell population density was approximately doubled. A model was derived from these cumulative data in an attempt to associate gravity-dependent, extracellular transport phenomena with unique changes observed in each specific phase of growth. It is suggested that a cumulative effect of gravity may have a significant impact on suspended cells via their fluid environment, where an immediate, direct influence of gravity might otherwise be deemed negligible.