Evidence of an inverted hexagonal phase in self-assembled phospholipid-DNA-metal complexes
Evidence of an inverted hexagonal phase in self-assembled phospholipid-DNA-metal complexes
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DOI：
10.1209/epl/i2004-10091-y
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发表时间：
2004-08-01
期刊：
影响因子：
--
通讯作者：
Weiss, TM
中科院分区：
文献类型：
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作者：
Francescangeli, O;Pisani, M;Weiss, TM
我们报告的第一个观察到的磷脂-DNA-金属络合物的一个倒置的六方相。当中性脂质二油酰磷脂酰乙醇胺（DOPE）、DNA和二价金属阳离子（Me 2 +; Me = Fe、Co、Mg、Mn）的水溶液混合时，这些三元复合物以自组装的方式形成，这代表了超分子化学的一个引人注目的例子。该结构来自同步加速器X射线衍射，由圆柱形DNA链组成，由中性脂质单层包裹并排列在二维六边形晶格上（H-11（c））。除了基本方面，DOPE-DNA-Me 2+复合物作为基因治疗应用中的有效非病毒递送系统可能具有极大的兴趣，因为其固有的低细胞毒性和潜在的高转染效率。
We report the first observation of an inverted hexagonal phase of phospholipid-DNA-metal complexes. These ternary complexes are formed in a, self-assembled manner when water solutions of neutral lipid dioleoylphosphatidylethanolamine (DOPE), DNA and divalent metal cations (Me2+; Me = Fe, Co, Mg, Mn) are mixed, which represents a striking example of supramolecular chemistry. The structure, derived from synchrotron X-ray diffraction, consists of cylindrical DNA strands coated by neutral lipid monolayers and arranged on a two-dimensional hexagonal lattice (H-11(c)). Besides the fundamental aspects, DOPE-DNA-Me2+ complexes may be of great interest as efficient nonviral delivery systems in gene therapy applications because of the low inherent cytotoxicity and the potential high transfection efficiency.