Pulpal Regeneration Following Allogenic Tooth Transplantation into Mouse Maxilla
Pulpal Regeneration Following Allogenic Tooth Transplantation into Mouse Maxilla
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DOI：
10.1002/ar.20831
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发表时间：
2009-04-01
影响因子：
2
通讯作者：
Ohshima, Hayato
中科院分区：
文献类型：
--
作者：
Unno, Hideki;Suzuki, Hironobu;Ohshima, Hayato
自体牙移植是目前牙科治疗缺牙的常用方法。然而，同种异体牙移植在临床应用中由于存在免疫排斥反应而面临诸多困难。本研究旨在通过5-溴-2'-脱氧尿苷（BrdU）和巢蛋白的免疫组化，以及抗酒石酸酸性磷酸酶（TRAP）的组织化学，阐明同种异体牙齿移植小鼠上颌后的牙髓再生。将2周龄小鼠右上第一磨牙（M1）取出，移植至同窝鼠和非同窝鼠的原窝内。牙移植可减弱牙髓组织中巢蛋白阳性反应，而牙髓组织术前对巢蛋白具有免疫反应性。术后第5-7天，第三牙本质在原有牙本质旁边开始形成，直到第14天，除一例在牙髓腔出现免疫排斥反应外，所有同窝组的病例都排列着巢蛋白阳性的成牙细胞样细胞。在非窝伴组中，直到第14天，除了第三牙本质的形成外，牙髓腔中还出现了骨样组织的形成。三级牙本质占38%，牙本质与骨样组织混合形成占23%（其余为免疫排斥）。有趣的是，即使在免疫排斥的情况下，牙髓腔被稀疏的结缔组织取代，牙周组织也恢复了。这些结果表明，同窝或非同窝的选择对成牙细胞系细胞的存活起决定性作用，免疫排斥反应不影响牙周再生。生态学报，29(2):557 -579,2009。(C) 2009 Wiley-Liss, Inc。
Autogenic tooth transplantation is now a common procedure in dentistry for replacing a missing tooth. However, there are many difficulties in clinical application of allogenic tooth transplantation because of immunological rejection. This study aims to clarify pulpal regeneration following allogenic tooth transplantation into the mouse maxilla by immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU) and nestin, and by the histochemistry for tartrate-resistant acid phosphatase (TRAP). The upper right first molar (M1) of 2-week-old mice was extracted and allografted in the original socket in both the littermate and non-littermate after the extraction of M1. Tooth transplantation weakened the nestin-positive reactions in the pulp tissue that had shown immunoreactivity for nestin before operation. On postoperative Days 5-7, tertiary dentin formation commenced next to the preexisting dentin where nestin-positive odontoblast-like cells were arranged in all cases of the littermate group until Day 14, except for one case showing immunological rejection in the pulp chamber. In the non-littermate group, bone-like tissue formation occurred in the pulp chamber in addition to tertiary dentin formation until Day 14. The rate of tertiary dentin was 38%, and the rate of the mixed form of dentin and bone-like tissue formation was 23% (the remainder was immunological rejection). Interestingly, the periodontal tissue recovered even in the case of immunological rejection in which the pulp chamber was replaced by sparse connective tissue. These results suggest that the selection of littermate or non-littermate is decisive for the survival of odontoblast-lineage cells and that the immunological rejection does not influence the periodontal regeneration. Anat Rec, 292:570-579, 2009. (C) 2009 Wiley-Liss, Inc.