Effects of monosodium glutamate-induced obesity in spontaneously hypertensive rats vs. Wistar Kyoto rats:: Serum leptin and blood flow to brown adipose tissue
Effects of monosodium glutamate-induced obesity in spontaneously hypertensive rats vs. Wistar Kyoto rats:: Serum leptin and blood flow to brown adipose tissue
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DOI：
10.1291/hypres.23.503
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发表时间：
2000-09-01
影响因子：
5.4
通讯作者：
Fujishima, M
中科院分区：
文献类型：
--
作者：
Iwase, M;Ichikawa, K;Fujishima, M
我们比较了谷氨酸钠(MSG)对自发性高血压大鼠(SHR)和血压正常的Wistar京都大鼠(WKY)所致下丘脑肥胖的影响。新生WKY组和SHR组大鼠每日腹腔注射4 mg/kg体重的味精，连续5d。在6月龄时，SHR的肥胖程度比WKY更严重，但在14月龄时两个品系的肥胖严重程度相似，味精治疗的SHR与味精治疗的WKY相比，高甘油三酯血症明显升高，尾套法测得的SHR的收缩压始终低于对照SHR，而WKY新生儿的血压不受味精治疗的影响。限制饮食对SHR体重的影响大于WKY对照组，前者也表现出促进酮体生成的作用，新生儿味精治疗取消了SHR体重的加速下降。在味精处理的肥胖大鼠中，血清瘦素浓度显著升高，但在对照组或味精处理组中，WKY和SHR之间没有发现差异。血清瘦素与Lee肥胖指数和肠系膜脂肪重量密切相关。激光多普勒血流仪测定，对照组和味精处理组大鼠肩关节间棕色脂肪组织(BAT)血流量在β(3)-肾上腺素受体激动剂BRL26830A作用下均显著增加。然而，血流量的反应不受味精处理或菌株差异的影响。目前的研究表明，WKY和SHR对新生儿味精治疗的反应存在一定的菌株差异。这些差异不能用血清瘦素水平或BAT中β(3)-肾上腺素能反应性的差异来解释。
We compared the effects of hypothalamic obesity induced by neonatal monosodium glutamate (MSG) treatment between spontaneously hypertensive rats(SHR) and normotensive Wistar Kyoto rats (WKY). Newborn WKY and SHR were injected intraperitoneally with 4 mg/kg body weight of MSG daily for 5 days. At 6 months of age, the obesity of SHR was more advanced than that of WKY, but at 14 months of age the severity of obesity was similar between the two strains, Hypertriglyceridemia was enhanced in MSG-treated SHR as compared with MSG-treated WKY, Systolic blood pressure measured by the tail-cuff method was consistently lower in MSG-treated SHR than in control SHR, whereas blood pressure was not affected by neonatal MSG treatment in WKY. Food restriction reduced body weight more in control SHR than in control WKY, with the former also showing enhanced ketogenesis, Neonatal MSG treatment abolished the accelerated reduction of body weight in SHR. Serum leptin concentration was markedly increased in MSG-treated obese rats, though no differences were seen between WKY and SHR in the control or MSG-treated groups. Serum leptin was closely correlated with both Lee obese index and mesenteric fat weight over the strain. Blood flow in interscapular brown adipose tissue (BAT) measured by Laser Doppler flowmetry was significantly increased in response to beta(3)-adrenoceptor agonist BRL26830A in both the control and MSG-treated rats. However, the response of blood flow was not affected by MSG treatment or strain difference. The present study demonstrated some strain differences in response to neonatal MSG treatment between WKY and SHR. These differences could not be explained by the difference in serum leptin level or beta(3)-adrenergic reactivity in BAT.