Cell-laden interpenetrating network hydrogels formed from methacrylated gelatin and silk fibroin via a combination of sonication and photocrosslinking approaches
Cell-laden interpenetrating network hydrogels formed from methacrylated gelatin and silk fibroin via a combination of sonication and photocrosslinking approaches
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通过超声处理和光交联方法相结合，由甲基丙烯酸明胶和丝素蛋白形成充满细胞的互穿网络水凝胶
DOI：
10.1016/j.msec.2019.01.079
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发表时间：
2019-06-01
影响因子：
7.9
通讯作者：
Liao, Xiaoling
中科院分区：
文献类型：
--
作者：
Xiao, Wenqian;Li, Jiale;Liao, Xiaoling
承载软组织的再生一直推动着生物活性水凝胶的研究和发展。水凝胶在承载组织中的应用面临的一个主要挑战是开发具有合适的生物功能和生物力学稳定性的水凝胶，使其接近于宿主组织。本文描述了一种新合成的基于明胶甲基丙烯酸酯(GelMA)和丝素蛋白(SF)的细胞负载互穿聚合物网络(IPN)水凝胶，它是通过连续超声和光交联形成的。实验结果表明，SF-GelMA IPN水凝胶具有较高的溶胀度、优异的力学性能、抗胶原酶降解能力和内部微结构疏松。此外，还可以通过改变预聚体成分来调整这些性能。二醋酸丙二钠(FDA/PI)染色和细胞计数试剂盒(CCK-8)分析表明，MC3T3-E1前成骨细胞附着在IPN水凝胶上并随后在其上增殖。此外，MC3T3-E1前成骨细胞的包裹和随后的细胞活力检测表明，整个IPN形成过程与细胞相容，并可以通过调节GelMA浓度来调节包裹细胞的生长，强调了它们对各种承载软组织工程的通用性。综上所述，本研究介绍了一类机械强度高、可调谐的细胞负载IPN水凝胶，该凝胶作为承载软组织工程支架具有很大的潜力。
The regeneration of load-bearing soft tissues has long driven the research and development of bioactive hydrogels. A major challenge facing the application of hydrogels to load-bearing tissues is the development of hydrogels with appropriate biological functionality and biomechanical stability that closely mimic the host tissue. In this paper, we describe a newly synthesized cell-laden interpenetrating polymer network (IPN) hydrogel based on gelatin methacrylate (GelMA) and silk fibroin (SF) that was formed via sequential sonication and photocrosslinking. The experimental results revealed that SF-GelMA IPN hydrogels exhibited high swelling ratios, excellent mechanical properties, resistance to enzymatic degradation by collagenase, and porous internal microstructures. Moreover, these properties could be tailored by changing the prepolymer components. MC3T3-E1 pre-osteoblasts attached to and subsequently proliferated on the IPN hydrogels, as demonstrated by fluorescein diacetate/propidium iodide (FDA/PI) staining and Cell Counting Kit-8 (CCK-8) analysis. In addition, the encapsulation of MC3T3-E1 pre-osteoblasts and a subsequent cell viability assay demonstrated that the entire IPN formation process was compatible with cells and that the growth of encapsulated cells could be tuned by adjusting the GelMA concentration, underlining their versatility for various load-bearing soft tissue engineering. Overall, this study introduces a class of mechanically robust and tunable cell-laden IPN hydrogels which have great potential as load-bearing soft tissue engineering scaffold.