CLONAL ANALYSIS IN THE CHICKEN RETINA REVEALS TANGENTIAL DISPERSION OF CLONALLY RELATED CELLS
CLONAL ANALYSIS IN THE CHICKEN RETINA REVEALS TANGENTIAL DISPERSION OF CLONALLY RELATED CELLS
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DOI：
10.1006/dbio.1994.1346
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发表时间：
1994-12-01
影响因子：
2.7
通讯作者：
CEPKO, CL
中科院分区：
文献类型：
--
作者：
FEKETE, DM;PEREZMIGUELSANZ, J;CEPKO, CL
利用逆转录病毒载体克隆分析鸡视网膜的发育情况。复制能力不足的逆转录病毒载体编码人类胎盘碱性磷酸酶、β -半乳糖苷酶或病毒核心蛋白，配对组合感染胚胎2.5-7.0天的视网膜祖细胞。在视网膜组织发生完成后，在胚胎发育后期对标记的克隆进行分析，鸡视网膜的早期可及性，加上其最终的大尺寸，导致标记的克隆比以前报道的其他物种大得多。这些克隆由多种细胞类型组成，支持了先前从其他脊椎动物身上得出的结论，即发育中的视网膜祖细胞是多能的。大多数来源于早期感染的克隆由多个紧密聚集的细胞阵列组成，并伴有分散的单个细胞。克隆复杂性和切向色散在周围视网膜大于中央视网膜，并随着感染年龄的增加而显著降低。这些观察结果表明，在视网膜发育早期，视神经杯形成后不久，有相当多的祖细胞混合。(C) 1994学术出版社，Inc.
Development of the chicken retina was investigated through clonal analysis using retroviral vectors. Replication-incompetent retroviral vectors encoding either human placental alkaline phosphatase, beta-galactosidase, or a viral core protein were used in paired combinations to infect retinal progenitor cells from Embryonic Days 2.5-7.0. Labeled clones were analyzed late in embryonic development after retinal histogenesis was complete,The early accessibility of the chicken retina, combined with its large final size, resulted in the labeling of much larger clones than had been reported previously in other species. The clones were composed of many cell types, supporting previous conclusions from other vertebrates that the progenitor cells of developing retina are multipotent. The majority of clones derived from early infections consisted of multiple tightly clustered arrays of cells accompanied by dispersed individual cells. Clonal complexity and tangential dispersion were greater in peripheral than central retina and decreased considerably with increasing age of infection. These observations suggest that early during retinal development, shortly after optic cup formation, there is considerable mixing of progenitor cells. (C) 1994 Academic Press, Inc.