Recurrent DNA copy number changes in 1q, 4q, 6q, 9p, 13q, 14q and 22q detected by comparative genomic hybridization in malignant mesothelioma.
Recurrent DNA copy number changes in 1q, 4q, 6q, 9p, 13q, 14q and 22q detected by comparative genomic hybridization in malignant mesothelioma.
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DOI：
10.1038/bjc.1997.91
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发表时间：
1997
影响因子：
8.8
通讯作者：
Knuutila S
中科院分区：
文献类型：
--
作者：
Björkqvist AM;Tammilehto L;Anttila S;Mattson K;Knuutila S
比较基因组杂交（CGH）分析进行了27人胸膜间皮瘤肿瘤标本，包括18个冷冻肿瘤和9个石蜡包埋的肿瘤，筛选的增益和损失的DNA序列。在27份标本中的15份中检测到拷贝数变化，每份标本的范围为1 - 8。平均而言，遗传物质的损失多于获得。DNA序列丢失最常见于9号染色体短臂（p21-pter），占异常标本的60%。其他异常染色体丢失主要发生在4号（q31.1-qter，20%）、6号（q22-q24，33%）、13号（33%）、14号（q24-qter，33%）和22号（q13，20%）长臂。在33%的异常标本中，发现1号染色体长臂（cent-qter）DNA序列增加。我们的分析是第一个全基因组筛选的增益和损失的DNA序列，使用比较基因组杂交在恶性胸膜间皮瘤肿瘤。本研究中检测到的反复出现的DNA序列变化表明相应的染色体区域很可能含有对间皮瘤的发生和进展重要的基因。
Comparative genomic hybridization (CGH) analyses were performed on 27 human pleural mesothelioma tumour specimens, consisting of 18 frozen tumours and nine paraffin-embedded tumours, to screen for gains and losses of DNA sequences. Copy number changes were detected in 15 of the 27 specimens with a range from one to eight per specimen. On average, more losses than gains of genetic material were observed. The loss of DNA sequences occurred most commonly in the short arm of chromosome 9 (p21-pter), in 60% of the abnormal specimens. Other losses among the abnormal specimens were frequently detected in the long arms of chromosomes 4 (q31.1-qter, 20%), 6 (q22-q24, 33%), 13 (33%),14 (q24-qter, 33%) and 22 (q13, 20%). A gain in DNA sequences was found in the long arm of chromosome 1 (cen-qter) in 33% of the abnormal specimens. Our analysis is the first genome-wide screening for gains and losses of DNA sequences using comparative genomic hybridization in malignant pleural mesothelioma tumours. The recurrent DNA sequence changes detected in this study suggest that the corresponding chromosomal areas most probably contain genes important for the initiation and progression of mesothelioma.