Biomineralization-Inspired Synthesis of Copper Sulfide-Ferritin Nanocages as Cancer Theranostics.
Biomineralization-Inspired Synthesis of Copper Sulfide-Ferritin Nanocages as Cancer Theranostics.
复制标题
生物矿化启发合成硫化铜-铁蛋白纳米笼作为癌症治疗学
DOI：
10.1021/acsnano.5b07521
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发表时间：
2016-03-22
期刊：
影响因子：
17.1
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Wang Z;Huang P;Jacobson O;Wang Z;Liu Y;Lin L;Lin J;Lu N;Zhang H;Tian R;Niu G;Liu G;Chen X
在纳米医学中，严格控制纳米颗粒的尺寸和形貌是至关重要的。蛋白质笼为无机纳米粒子的模板合成提供了重要的潜力。在这项研究中，我们成功地合成超小硫化铜（CuS）纳米粒子的铁蛋白（Fn）纳米笼的空腔内的仿生合成方法。均匀的CuS-Fn纳米笼（CuS-Fn NCs）具有较强的近红外吸收和较高的光热转换效率。在定量比率光声成像（派）中，CuS-Fn NC表现出上级光声断层扫描改善整个肿瘤的实时体内派。随着放射性核素64 Cu的掺入，64 CuS-Fn NC也用作与游离铜相比具有更高肿瘤蓄积的优异PET成像剂。在派和PET的指导下，将CuS-Fn纳米粒应用于光热治疗，在体外和体内均获得了良好的生物相容性和上级肿瘤治疗效果。结果表明，生物启发的多功能CuS-Fn NCs具有作为临床可翻译的癌症治疗诊断学的潜力，并且可以在实验和临床环境中为癌症光热疗法提供非侵入性、高灵敏度和定量的体内指导方法。
It is essential to control the size and morphology of nanoparticles strictly in nanomedicine. Protein cages offer significant potential for templated synthesis of inorganic nanoparticles. In this study, we successfully synthesized ultrasmall copper sulfide (CuS) nanoparticles inside the cavity of ferritin (Fn) nanocages by a biomimetic synthesis method. The uniform CuS–Fn nanocages (CuS–Fn NCs) showed strong near-infrared absorbance and high photothermal conversion efficiency. In quantitative ratiometric photoacoustic imaging (PAI), the CuS–Fn NCs exhibited superior photoacoustic tomography improvements for real-time in vivo PAI of entire tumors. With the incorporation of radionuclide 64Cu, 64CuS–Fn NCs also served as an excellent PET imaging agent with higher tumor accumulation compared to free copper. Following the guidance of PAI and PET, CuS–Fn NCs were applied in photothermal therapy to achieve superior cancer therapeutic efficiency with good biocompatibility both in vitro and in vivo. The results demonstrate that the bioinspired multifunctional CuS–Fn NCs have potential as clinically translatable cancer theranostics and could provide a noninvasive, highly sensitive, and quantitative in vivo guiding method for cancer photothermal therapies in experimental and clinical settings.