(Na+-K+)-ATPase activity and ouabain-binding sites in the cerebral cortex of young and aged Fischer-344 rats.
(Na+-K+)-ATPase activity and ouabain-binding sites in the cerebral cortex of young and aged Fischer-344 rats.
复制标题
年轻和老年 Fischer-344 大鼠大脑皮层的 (Na -K )-ATP 酶活性和哇巴因结合位点。
作者：
LaManna,JC;Doull,G;McCracken,K;Harik,SI
(Na（+-K+）-ATP酶是负责可兴奋组织中离子稳态的酶。因此，这种酶的活性对于维持大脑皮层的最佳功能至关重要。虽然脑氧化代谢异常已被描述在老化，这些异常似乎并不与（Na+-K+）-ATP酶的体外酶特性的变化。具体而言，3月龄和28月龄Fischer-344大鼠大脑皮层制备物在以下方面无显著差异：（i）与[3 H]-哇巴因的高亲和力结合，（ii）哇巴因结合位点的数量，或（iii）K+ p-硝基苯磷酸酶活性。
(Na+-K+)-ATPase is an enzyme responsible for ionic homeostasis in excitable tissues. Thus, the activity of this enzyme is essential for the maintenance of optimal function of the cerebral cortex. Although abnormalities of cerebral oxidative metabolism have been described in aging, these abnormalities do not appear to be associated with changes in the in vitro enzyme characteristics of (Na+-K+)-ATPase. Specifically, there were no significant differences between preparations from the cerebral cortex of 3- and 28-month-old Fischer-344 rats in their: (i) high-affinity binding to [3H]-ouabain, (ii) number of ouabain-binding sites, or (iii) K+p-nitrophenylphosphatase activity.