Specific diagnosis of progressive multifocal leukoencephalopathy by polymerase chain reaction.
Specific diagnosis of progressive multifocal leukoencephalopathy by polymerase chain reaction.
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聚合酶链反应特异性诊断进行性多灶性白质脑病。
DOI：
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发表时间：
1994
影响因子：
6.4
通讯作者：
G. Hunsmann
中科院分区：
文献类型：
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作者：
T. Weber;R. Turner;S. Frye;B. Ruf;J. Haas;E. Schielke;H. Pohle;W. Lüke;W. Lüer;K. Felgenhauer;G. Hunsmann
使用聚合酶链反应 (PCR) 对 28 名进行性多灶性白质脑病 (PML) 患者的 34 份脑脊液 (CSF) 样本进行了分析。作为对照，对来自 82 名人类免疫缺陷病毒 1 型 (HIV-1) 感染患者和 1 名 HIV-1 阴性患者的 116 个样本进行了评估。在HIV-1阳性患者中，23人患有脑弓形体病，10人患有HIV白质脑病，49人患有其他神经系统并发症。在苯酚-氯仿-异戊醇提取之前添加载体 DNA，脑脊液中 JC 病毒 (JCV) DNA 的检测结果增加了 10 倍。来自 VP1/大 T 区域的引物对 JC 26/29 的检测限为 10(5) 个 JCV DNA 分子/100 µL。位于大T基因区域的引物对JC 36/39具有100倍的检测下限。对于 JC 26/29，敏感性为 43% (12/28)，特异性为 100%。使用 JC 36/39，敏感性增加至 82% (23/28)，并且未观察到假阳性结果。脑脊液 PCR 分析对 PML 的诊断有很大帮助。
Using polymerase chain reaction (PCR), 34 cerebrospinal fluid (CSF) samples from 28 patients with progressive multifocal leukoencephalopathy (PML) were analyzed. As controls, 116 samples were evaluated from 82 human immunodeficiency virus type 1 (HIV-1)-infected patients and 1 HIV-1-negative patient. Of the HIV-1-positive patients, 23 had cerebral toxoplasmosis, 10 had HIV leukoencephalopathy, and 49 had other neurologic complications. Detection of JC virus (JCV) DNA in CSF was increased 10-fold by the addition of carrier DNA before phenol-chloroform-isoamyl alcohol extraction. The primer pair JC 26/29, from the VP1/large T region, had a limit of detection of 10(5) JCV DNA molecules/100 microL. The primer pair JC 36/39, located in the large T gene region, had a 100-fold lower limit of detection. With JC 26/29, the sensitivity was 43% (12/28) and specificity was 100%. Using JC 36/39, sensitivity increased to 82% (23/28), and false-positive results were not observed. Diagnosis of PML is greatly aided by PCR analysis of CSF.