Regulation of fatty acid oxidation in chicken (Gallus gallus): Interactions between genotype and diet composition
Regulation of fatty acid oxidation in chicken (Gallus gallus): Interactions between genotype and diet composition
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DOI：
10.1016/j.cbpb.2009.02.012
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发表时间：
2009-06-01
影响因子：
2.2
通讯作者：
Tesseraud, S.
中科院分区：
文献类型：
--
作者：
Collin, A.;Swennen, Q.;Tesseraud, S.
为了探索导致鸡过度肥胖的机制，我们研究了与基因相关的体脂和日粮组成对脂肪酸氧化的调节。测定了低脂/高蛋白饲料和等能高脂/低蛋白饲料(HF/LP)饲养的遗传瘦肉鸡和肥鸡肝脏和腓肠肌中线粒体能量代谢相关蛋白的表达和/或活性。肌肉中肉碱-蛋白转移酶1亚型(M-CPT1)和PPARβ/Delta在脂肪中的表达高于瘦肉鸡。这也在肝脏中观察到，尽管只在M-CPTI的HF/LP饮食中观察到。这可能会刺激肥鸡体内线粒体的脂肪添加氧化。肝脏和肌肉CPT-1肝脏异构体、肌肉细胞色素-c-氧化酶mRNA表达和β-羟基酰基辅酶A脱氢酶活性的上调，表明HF/LP饮食对脂肪酸的利用更高。PPARβ/Delta和PGC-1α分别对肌肉和肝脏的脂肪酸氧化有抑制作用。禽解偶联蛋白(AvUCP)基因的调控具有组织依赖性。它主要在肌肉中表达，在脂肪和饲喂HF/LP的鸡中受到刺激，这可能与肥胖动物对肌肉抗氧化途径的特殊需要有关。在肝脏中，它的脂肪含量低于瘦肉鸡，其潜在功能仍有待阐明。(C)2009 Elsevier Inc.保留所有权利。
To explore the mechanisms leading to excessive adiposity in chicken, we investigated the regulation of fatty acid oxidation depending on genotype-related body fatness and diet composition. mRNA expression and/or activity of proteins involved in mitochondrial energy metabolism were measured in liver and gastrocnemius muscle of genetically lean or fat chickens reared on a low-fat/high-protein diet or an isoenergetic high-fat/low-protein diet (HF/LP). Muscle expressions of the muscle isoform of carnitine-paimitoyltransferase 1 (M-CPT1) and PPAR beta/delta were higher in fat than in lean chickens. This was also observed in liver, although only with the HF/LP diet for M-CPTI. This could stimulate mitochondrial fatty add oxidation in fat chickens. Up-regulations of liver and muscle CPT-1 hepatic isoform, and muscle cytochrome-c-oxidase mRNA expressions, and of beta-hydroxyacyl-CoA-dehydrogenase activities suggest higher fatty acid utilization with the HF/LP diet. PPAR beta/delta and PGC-1 alpha could control fatty acid oxidation in muscle and liver, respectively. Regulation of avian uncoupling protein (avUCP) mRNA was tissue-dependent. Predominantly expressed in muscle, it was stimulated in fat and in HF/LP-fed chickens, where it could be associated to the special need in muscle anti-oxidant pathways of fatter animals. In liver it was lower in fat than in lean chickens, and its potential function remains to be clarified. (C) 2009 Elsevier Inc. All rights reserved.