INTERMEDIATES IN THE FOLDING OF THE MEMBRANE-PROTEIN BACTERIORHODOPSIN
INTERMEDIATES IN THE FOLDING OF THE MEMBRANE-PROTEIN BACTERIORHODOPSIN
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DOI：
10.1038/nsb0295-139
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发表时间：
1995-02-01
期刊：
影响因子：
--
通讯作者：
KHORANA, HG
中科院分区：
文献类型：
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作者：
BOOTH, PJ;FLITSCH, SL;KHORANA, HG
蛋白质在脂质双层内的组装对于生物膜的生物发生和功能是必不可少的。然而，人们对组装的基本机制知之甚少，也不清楚是否有可能在体内或体外观察到完整的膜蛋白的个别折叠步骤。本文利用荧光光谱跟踪细菌视紫红质在洗涤剂/脂肪混合胶束中折叠的时间过程。检测到瞬时折叠中间体，视网膜发色团的结合发生在晚期，当它形成脱辅基蛋白中间体时。
Assembly of proteins within lipid bilayers is essential for the biogenesis and function of biological membranes. Little is known, however, about the underlying mechanism of assembly, and it is not clear whether it is possible to observe individual folding steps for integral membrane proteins either in vivo or in vitro. Fluorescence spectroscopy is used here to follow the time course of folding events for bacteriorhodopsin in mixed detergent/lipid micelles. Transient folding-intermediates are detected and binding of the retinal chromophore occurs at a late stage, when it kinds to an apoprotein intermediate.