Atg5 Regulates Selective Autophagy of the Parental Macronucleus during Tetrahymena Sexual Reproduction.
Atg5 Regulates Selective Autophagy of the Parental Macronucleus during Tetrahymena Sexual Reproduction.
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Atg5 调节四膜虫有性生殖过程中亲本大核的选择性自噬
作者：
Bo T;Kang Y;Liu Y;Xu J;Wang W
核自噬是一种重要的选择性自噬过程。嗜热四膜虫在有性生殖过程中发生性发育微核的选择性自噬和亲代大核的程序性核降解。核选择性自噬的分子调控机制尚不清楚。本研究从T.嗜热菌Atg5在性发育早期定位于细胞质，在性发育晚期定位于paMAC。在此阶段，MIC的减数分裂产物的降解被延迟atg5i突变体。此外，paMAC异常增大且延迟或未能降解。Atg8.2的表达水平和脂化在突变体中显著降低。这些结果表明Atg5可能通过调节Atg8.2参与了四膜虫paMAC选择性自噬的调控。
Nuclear autophagy is an important selective autophagy process. The selective autophagy of sexual development micronuclei (MICs) and the programmed nuclear degradation of parental macronucleus (paMAC) occur during sexual reproduction in Tetrahymena thermophila. The molecular regulatory mechanism of nuclear selective autophagy is unclear. In this study, the autophagy-related protein Atg5 was identified from T. thermophila. Atg5 was localized in the cytoplasm in the early sexual-development stage and was localized in the paMAC in the late sexual-development stage. During this stage, the degradation of meiotic products of MIC was delayed in atg5i mutants. Furthermore, paMAC was abnormally enlarged and delayed or failed to degrade. The expression level and lipidation of Atg8.2 significantly decreased in the mutants. All these results indicated that Atg5 was involved in the regulation of the selective autophagy of paMAC by regulating Atg8.2 in Tetrahymena.
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