Focal cone ERGs of rhodopsin Pro347Leu transgenic rabbits.
Focal cone ERGs of rhodopsin Pro347Leu transgenic rabbits.
复制标题
视紫红质 Pro347Leu 转基因兔的焦锥 ERG。
DOI：
10.1016/j.visres.2013.08.006
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Terasaki H
中科院分区：
文献类型：
--
作者：
Ueno S;Koyasu T;Kominami T;Sakai T;Kondo M;Yasuda S;Terasaki H
本实验室建立了视网膜色素变性动物模型——视紫红质P347L转基因（Tg）家兔。本研究的目的是确定该兔模型视网膜变性过程中视网膜焦点区域的特性。为了做到这一点，我们记录了野生型（WT）和Tg兔在3、6和12个月时的局灶性心电图。采用15°刺激点从视觉条纹中心和周围四个区域引出焦点电刺激。我们发现Tg家兔的焦锥ERG b波振幅和振荡电位（OPs）随着年龄的增加而逐渐下降，在12个月时几乎不可记录。5个区域记录的Tg家兔b波无显著的区域差异。各记录区OPs/b波比均高于WT家兔，且OPs/b波比保存较好。在3个月和6个月大时，视盘上方区域记录的总OPs振幅明显大于其他区域，最可能来自无突细胞和/或神经节细胞。这说明在该兔模型中，光感受器变性后视网膜内神经元的改变并不均匀。
A rhodopsin P347L transgenic (Tg) rabbit, a model of retinitis pigmentosa, has been generated in our laboratory. The purpose of this study was to determine the properties of focal areas of the retina in this rabbit model during the course of retinal degeneration. To accomplish this, we recorded focal ERGs from wild-type (WT) and Tg rabbits at ages 3, 6, and 12 months. A 15° stimulus spot was used to elicit the focal ERGs from the center of the visual streak and from four surrounding areas. We found that the amplitudes of the focal cone ERG b-waves and oscillatory potentials (OPs) of the Tg rabbits in the five areas decreased progressively with increasing age and became almost non-recordable at 12 months. There were no significant regional differences in the b-waves of Tg rabbits recorded from the 5 areas. The amplitudes of the OPs were better preserved than the b-waves and the OPs/b-wave ratio was higher than that in WT rabbits at every recording area. The summed OPs amplitudes, which most likely originate from the amacrine and/or ganglion cells, recorded from the area superior to the optic disc was significantly larger than that from other areas at 3- and 6-months-old. This indicated that the inner retinal neurons were not altered equally after photoreceptor degeneration in this rabbit model.