Enhanced (Na+K)-ATPase activity and expression in mouse brain after chronic ethanol administration.
Enhanced (Na+K)-ATPase activity and expression in mouse brain after chronic ethanol administration.
复制标题
长期乙醇给药后小鼠大脑中 (Na K)-ATP 酶活性和表达增强。
DOI：
10.1023/a:1022470019604
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发表时间：
1997
影响因子：
4.4
通讯作者：
Sun,AY
中科院分区：
文献类型：
--
作者：
Chen,Y;Wixom,PM;Sun,AY
一般假设认为，乙醇的主要作用方式是改变膜结构和功能，包括神经传递和信号转导所必需的受体和离子通道的构象。然而，乙醇在生理条件下是否影响(Na+K)-ATP酶的问题仍然悬而未决。在这项研究中，成年小鼠每天接受 5 g/kg 剂量的乙醇治疗，持续 28 天。从脑中分离 RNA，并使用 Northern 印迹分析测定 (Na+K)-ATPase mRNA 水平。我们发现，与对照组相比，长期饮酒组的 (Na+K)-ATPase α 亚基表达增加。长期施用乙醇后突触体质膜中蛋白质磷酸化增加以及该酶的比活性增加进一步证实了这一结果。因此，我们证明乙醇可能直接影响体内的（Na+K）-ATP酶，通过适应性机制导致该酶的合成增加。
It is the general hypothesis that the primary mode of action of ethanol is the alteration of membrane structure and function including the conformation of receptors and ion channels essential for neurotransmission and signal transduction. However, the issue of whether ethanol affects (Na+K)-ATPase under physiological conditions remains unsettled. In this study, adult mice were treated with a daily dose of 5 g/kg of ethanol for 28 days. The RNA was isolated from brain and the (Na+K)-ATPase mRNA level was determined using Northern blot analysis. We have found an increased expression of (Na+K)-ATPase α-subunit in the chronically treated alcohol group as compared with that of controls. This result was further substantiated by increased protein phosphorylation as well as increased specific activity of this enzyme in the synaptosomal plasma membrane after chronic ethanol administration. Thus we have demonstrated that ethanol may directly affect (Na+K)-ATPase in vivo, leading to the increased synthesis of this enzyme through adaptive mechanisms.