Shear stress alters pleural mesothelial cell permeability in culture.
Shear stress alters pleural mesothelial cell permeability in culture.
复制标题
剪切应力改变培养物中的胸膜间皮细胞的通透性。
DOI：
10.1152/jappl.1996.81.1.448
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Grotberg,JB
中科院分区：
文献类型：
--
作者：
Waters,CM;Glucksberg,MR;Depaola,N;Chang,J;Grotberg,JB
肺对胸壁的滑动运动在胸膜间隙产生剪切应力，根据呼吸速率可高达60 dyn/cm2。这种剪切应力可能影响排列在肺胸膜间隙（内脏胸膜）和胸壁（胸膜壁层）上的间皮细胞。当在平行板流动室中暴露于剪切应力（17 dyn/cm2） 22小时时，大鼠内脏胸膜间皮细胞的形态没有改变，也没有沿着流动方向排列，这与牛主动脉内皮细胞的形状变化形成了对比。通过在多孔微载体珠上培养间皮细胞，我们用细胞-柱色谱法测定了细胞在不同流速下的通透性。当流量从0.9 ml/min增加到3.5 ml/min（对应平均剪应力4.7 ~ 18.4 dyn/cm2）时，对荧光素钠和氰钴胺素的渗透率分别从8.2 +/- 1.0和7.8 +/- 0.7 × 10(-5) cm/s增加到22.5 +/- 1.2和21.8 +/- 3.0 × 10(-5) cm/s。当流量减少时，渗透率恢复到基线值。细胞松弛素D刺激了渗透性的增加，而随后的剪切应力的增加并没有增强这种增加。这些结果表明，间皮细胞的屏障功能对流体剪切应力的变化具有响应性。
The sliding motion of the lung against the chest wall creates a shear stress in the pleural space, which can be as high as 60 dyn/cm2, depending on the respiration rate. Such shear stresses may affect the mesothelial cells that line the pleural space on the lung (visceral pleura) and chest wall (parietal pleura). When exposed to shear stress (17 dyn/cm2) in a parallel-plate flow chamber for 22 h, rat visceral pleura mesothelial cells were not altered morphologically and did not align in the direction of flow, in contrast to the shape changes observed for bovine aortic endothelial cells. By using mesothelial cells cultured on porous microcarrier beads, we measured the permeability of the cells at different flows in a cell-column chromatography assay. The permeabilities to sodium fluorescein and cyanocobalamin increased from 8.2 +/- 1.0 and 7.8 +/- 0.7 x 10(-5) cm/s to 22.5 +/- 1.2 and 21.8 +/- 3.0 x 10(-5) cm/s, respectively, when the flow was increased from 0.9 to 3.5 ml/min (corresponding to average shear stresses of 4.7-18.4 dyn/cm2). The permeabilities returned to baseline values when the flow was reduced. Cytochalasin D stimulated an increase in permeability that was not augmented by a subsequent increase in shear stress. These results suggest that the barrier function of mesothelial cells is responsive to changes in fluid shear stress.