Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
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DOI：
10.1063/1.4991005
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发表时间：
2017-07-01
期刊：
影响因子：
3.2
通讯作者：
Lee, Gwo-Bin
中科院分区：
文献类型：
--
作者：
Gopinathan, Priya;Hung, Lien-Yu;Lee, Gwo-Bin
本文开发了一种能够自动识别胆管癌（CCA）细胞特异性适配体的集成微流控系统。开发的系统能够通过芯片上的指数富集（Cell-SELEX）过程进行基于细胞的配体系统进化，并且仅需要6轮Cell-SELEX来鉴定高特异性适配体；与传统的SELEX相比，这代表了速度的显著提高，通常需要15-20轮。利用开发的微流控芯片，成功筛选了3个CCA细胞适配体（SNU-478细胞适配体1个，HuCCT-1细胞适配体2个）。这种自动化系统可以被修改，以发现针对其他癌细胞的适体探针，从而允许早期诊断，从而潜在地改善预后。AIP出版社出版。
An integrated microfluidic system capable of automatically identifying aptamers specific to cholangiocarcinoma (CCA) cells was developed herein. The developed system was capable of performing cell-based systematic evolution of ligands via an exponential enrichment (Cell-SELEX) process on-chip, and only six rounds of Cell-SELEX were required to identify high specificity aptamers; this represents a significant improvement in speed over conventional SELEX, in which 15-20 rounds are typically required. Using the microfluidic chip developed, three aptamers specific to CCA cells (one for SNU-478 cells and two for HuCCT-1 cells) were successfully screened. This automated system could be modified to uncover aptamer probes against other cancer cells, thereby allowing for earlier diagnosis and consequently a potentially improved prognosis. Published by AIP Publishing.