A new species, Embellisia astragali sp. nov., causing standing milk-vetch disease in China
A new species, Embellisia astragali sp. nov., causing standing milk-vetch disease in China
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DOI：
10.1080/15572536.2007.11832565
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发表时间：
2007-05
期刊：
影响因子：
2.8
通讯作者：
Yanzhong Li;Z. Nan
中科院分区：
文献类型：
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作者：
Yanzhong Li;Z. Nan
本文报道了一新种，即恩氏丝毛孢属（Emellisia sp. nov.）从沙打旺（Astragalus adsurgens）的坏死叶、叶柄和茎中分离得到。对E.并与四个相似种E. abundans，E. oxytropis、棘豆E. phragmospora和E. telluster。该菌在茎和叶片中细胞间生长，在叶中细胞内生长。它从大多数种子来源中分离出来，频率为0.1- 44.6%。25 ℃时，马铃薯-胡萝卜琼脂、马铃薯-葡萄糖琼脂、小麦干草煎液（WHDA）和V8上的菌落生长速率分别为0.48、0.32、0.68和0.27 mm d−1。在WHDA上菌落生长的最适温度为20-25 ℃，高于30 ℃时没有生长。用E.通过将整个根和修剪的根浸在分生孢子悬浮液中并将悬浮液倒在已经种植了两株幼苗的土壤表面上来进行接种。20 wk后，分别有66.5%、62.1%和85.0%的植株发病，24.1%、20.7%和17.5%的植株死亡。症状包括更多侧枝的发育，具有小的、弯曲的、坏死的和变黄的幼叶，植物发育不良，红棕色病变，茎布朗宁，枯死，枝枯病，冠腐病，根黑腐病和植物死亡。这是首次报道豆科植物上的一种致病性Emellisia。
A new species, Embellisia astragali sp. nov., is described from necrotic leaves, petioles and stems of Astragalus adsurgens in China. The morphology of E. astragali is compared and contrasted to that of four similar species, E. abundans, E. oxytropis, E. phragmospora and E. telluster. The fungus grew intercellularly in stems and leaf blades and intracellularly in leaves. It was isolated from most sources of seeds at frequencies of 0.1–44.6%. Growth rates of colonies on potato-carrot agar, potato-dextrose agar, wheat hay decoction (WHDA) and V8 at 25 C were 0.48, 0.32, 0.68 and 0.27 mm d−1, respectively. The optimal temperature for colony growth on WHDA was 20–25 C, and no growth was measured above 30 C. Five week old standing milk-vetch seedlings were inoculated with E. astragali by dipping whole roots and pruned roots in a conidial suspension and pouring the suspension onto the soil surface in which two seedlings had been planted. After 20 wk 66.5%, 62.1% and 85.0% plants were diseased and 24.1% , 20.7% and 17.5% plants were dead, respectively. Symptoms included the development of more side shoots with small, curved, necrotic and yellowed young leaves, plant stunting, reddish brown lesions, stem browning, dieback, shoot blight, crown rot, root black rot and plant death. This is first report of a pathogenic Embellisia on legumes.