Depth-resolved imaging of photosensitizer in the rodent brain using fluorescence laminar optical tomography.
Depth-resolved imaging of photosensitizer in the rodent brain using fluorescence laminar optical tomography.
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DOI：
10.1117/1.jbo.25.9.096007
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发表时间：
2020-09
影响因子：
3.5
通讯作者：
Huang HC
中科院分区：
文献类型：
--
作者：
Gaitan B;Inglut CT;Liu Y;Chen Y;Huang HC
重要性：以前的研究已经进行了图像光敏剂在某些器官和肿瘤使用荧光层光学断层扫描。目前，还没有发表的工作，以定量地比较信号补偿的荧光层光学断层扫描与二维（2-D）成像组织。目的：本研究的目的是量化的好处，荧光层光学断层扫描持有超过2-D成像。我们比较了荧光层光学断层扫描与最大强度投影成像模拟2-D成像，因为这将是最相似和最严格的比较。方法：将填充有光敏剂的毛细管放置在体模和离体啮齿动物脑中，获得荧光层光学断层扫描和最大强度投影图像。对该方向上的信号损失进行量化和比较，以了解哪种方法可以更好地补偿由组织衰减引起的信号损失。结果如下：结果表明，我们可以重建毛细血管充满苯并卟啉衍生物光敏剂忠实地在幻影和离体啮齿动物脑组织中使用荧光层光学断层扫描。我们进一步证明，与最大强度投影成像相比，我们可以更好地补偿信号损失。结论：与最大强度投影成像相比，使用荧光层状光学断层成像（FLOT），当在离体啮齿动物脑组织中成像时，可以补偿组织的更深部分中的信号损失。
Significance: Previous studies have been performed to image photosensitizers in certain organs and tumors using fluorescence laminar optical tomography. Currently, no work has yet been published to quantitatively compare the signal compensation of fluorescence laminar optical tomography with two-dimensional (2-D) imaging in tissues. Aim: The purpose of this study is to quantify the benefit that fluorescence laminar optical tomography holds over 2-D imaging. We compared fluorescence laminar optical tomography with maximum intensity projection imaging to simulate 2-D imaging, as this would be the most similar and stringent comparison. Approach: A capillary filled with a photosensitizer was placed in a phantom and ex vivo rodent brains, with fluorescence laminar optical tomography and maximum intensity projection images obtained. The signal loss in the direction was quantified and compared to see which methodology could compensate better for signal loss caused by tissue attenuation. Results: The results demonstrated that we can reconstruct a capillary filled with benzoporphyrin derivative photosensitizers faithfully in phantoms and in ex vivo rodent brain tissues using fluorescence laminar optical tomography. We further demonstrated that we can better compensate for signal loss when compared with maximum intensity projection imaging. Conclusions: Using fluorescence laminar optical tomography (FLOT), one can compensate for signal loss in deeper parts of tissue when imaging in ex vivo rodent brain tissue compared with maximum intensity projection imaging.