Expression of receptor for ecotropic murine leukemia virus on hematopoietic cells
Expression of receptor for ecotropic murine leukemia virus on hematopoietic cells
复制标题
亲嗜性鼠白血病病毒受体在造血细胞上的表达
作者：
T. Suzuki;S. Aizawa;H. Ikeda
概述.通过杆状病毒表达系统产生由Friend MuLV包膜糖蛋白的表面（SU）亚基和绿色荧光蛋白（GFP）组成的融合蛋白（F-SU/GFP）。 F-SU/GFP特异性结合表达mCAT-1（嗜亲性小鼠白血病病毒（Eco-MuLV）受体）的哺乳动物组织培养细胞。基于细胞吸收F-SU/GFP的能力测量造血细胞上mCAT-1的表达水平。在易受Eco-MuLV感染的BALB/c小鼠中，所有造血细胞亚群均吸收F-SU/GFP，在TER 119+成红细胞中观察到最显著的吸收。来自C4 W和AKR小鼠的造血细胞不像BALB/c细胞那样容易吸收F-SU/GFP，可能是由于这些小鼠中内源性Eco-MuLV的包膜糖蛋白的表达。尽管AKR小鼠对Eco-MuLV感染敏感，而C4 W小鼠具有抗性，但这些小鼠在F-SU/GFP吸收能力方面没有表现出明显差异。
Summary. A fusion protein (F-SU/GFP) which is comprised of the surface (SU) subunit of the Friend MuLV envelope glycoprotein and the green fluorescence protein (GFP) was generated by a baculovirus expression system. The F-SU/GFP specifically bound to mammalian tissue cultured cells expressing the mCAT-1, the receptor for ecotropic murine leukemia virus (Eco-MuLV). The expression level of mCAT-1 on hematopoietic cells was measured based on the capacity of cells to absorb the F-SU/GFP. In BALB/c mice susceptible to Eco-MuLV infection, all hematopoietic cell subpopulations absorbed the F-SU/GFP with the most prominent absorption observed in the TER119+ erythroblasts. Hematopoietic cells from C4W and AKR mice did not absorb the F-SU/GFP as readily as the BALB/c cells, probably due to expression of the envelope glycoproteins of endogenous Eco-MuLVs in these mice. Although AKR mice are susceptible to Eco-MuLV infection while C4W mice are resistant, these mice showed no apparent difference in the F-SU/GFP-absorbing capacity.
鼠共嗜性逆转录病毒受体的 N-连接糖基化在病毒感染的细胞中发生改变。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Kim,JW;Cunningham,JM
通讯作者：
Cunningham,JM
亲嗜性鼠逆转录病毒对双功能细胞表面受体/碱性氨基酸转运蛋白的影响。
DOI：
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发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Wang,H;Dechant,E;Kavanaugh,M;North,RA;Kabat,D
通讯作者：
Kabat,D