Visual pattern memory requires foraging function in the central complex of Drosophila.
Visual pattern memory requires foraging function in the central complex of Drosophila.
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DOI：
10.1101/lm.873008
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发表时间：
2008-03
影响因子：
2
通讯作者：
Zhipeng Wang;Yufeng Pan;Weizhe Li;Huoqing Jiang;Lazaros Chatzimanolis;Jianhong Chang;Zhefeng Gong;Li Liu
中科院分区：
文献类型：
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作者：
Zhipeng Wang;Yufeng Pan;Weizhe Li;Huoqing Jiang;Lazaros Chatzimanolis;Jianhong Chang;Zhefeng Gong;Li Liu
觅食 (for) 基因编码环鸟苷-3',5'-单磷酸 (cGMP) 依赖性蛋白激酶 (PKG)，其在果蝇食物搜索行为中的作用已得到深入研究。然而，它在其他复杂行为中的功能尚未得到很好的表征。在这里，我们在果蝇中进行实验表明，for 基因在操作性视觉学习范式中是必需的。自然变种漫游者 (for(R)) 的视觉模式记忆正常，但另一种 PKG 水平较低的自然变种漫游者 (for(S)) 的视觉模式记忆受损。 for(S) 果蝇的记忆缺陷可以通过扇形体内 for 的组成型或成体有限表达来挽救。有趣的是，我们发现当 for 在椭球体中表达时，也会发生这种救援。此外，在扇形体的第五层中表达 for 可以恢复对图案参数“高程”的足够记忆，但不能恢复对“轮廓方向”的足够记忆，而在椭圆体中表达 for 则可以恢复对这两个参数的足够记忆。我们的研究定义了一个果蝇模型，以进一步了解 cGMP-PKG 信号在联想学习/记忆中的作用以及这种依赖视觉模式记忆的神经回路。
The role of the foraging (for) gene, which encodes a cyclic guanosine-3',5'-monophosphate (cGMP)-dependent protein kinase (PKG), in food-search behavior in Drosophila has been intensively studied. However, its functions in other complex behaviors have not been well-characterized. Here, we show experimentally in Drosophila that the for gene is required in the operant visual learning paradigm. Visual pattern memory was normal in a natural variant rover (for(R)) but was impaired in another natural variant sitter (for(S)), which has a lower PKG level. Memory defects in for(S) flies could be rescued by either constitutive or adult-limited expression of for in the fan-shaped body. Interestingly, we showed that such rescue also occurred when for was expressed in the ellipsoid body. Additionally, expression of for in the fifth layer of the fan-shaped body restored sufficient memory for the pattern parameter "elevation" but not for "contour orientation," whereas expression of for in the ellipsoid body restored sufficient memory for both parameters. Our study defines a Drosophila model for further understanding the role of cGMP-PKG signaling in associative learning/memory and the neural circuit underlying this for-dependent visual pattern memory.