PLD1 is overexpressed in an ER-negative MCF-7 cell line variant and a subset of phospho-Akt-negative breast carcinomas.
PLD1 is overexpressed in an ER-negative MCF-7 cell line variant and a subset of phospho-Akt-negative breast carcinomas.
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DOI：
10.1038/sj.bjc.6603926
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发表时间：
2007-09-17
影响因子：
8.8
通讯作者：
Arcaro, K. F.
中科院分区：
文献类型：
--
作者：
Gozgit, J. M.;Pentecost, B. T.;Marconi, S. A.;Ricketts-Loriaux, R. S. J.;Otis, C. N.;Arcaro, K. F.
我们使用了人雌激素受体（ER）阳性的MCF - 7细胞系的一种新型变体TMX2 - 28作为研究乳腺癌的模型。TMX2 - 28细胞未检测到雌激素受体的mRNA或蛋白质表达水平，且表达基底细胞角蛋白（CKs）5、14和17。TMX2 - 28与其亲本细胞系MCF - 7之间的cDNA微阵列比较鉴定出1402个差异表达的转录本，其中之一是磷脂酶D1（PLD1）。通过实时逆转录 - 聚合酶链反应，我们证实TMX2 - 28细胞中PLD1的mRNA水平比MCF - 7细胞高10倍。接下来我们检测了人乳腺癌中PLD1的表达。在表达高水平基底细胞角蛋白5和/或17的mRNA的乳腺肿瘤中，磷脂酶D1的mRNA水平较高，但在雌激素受体阴性的肿瘤中，PLD1的mRNA水平并没有显著升高。通过免疫组化检测的42个乳腺肿瘤中有10个（24%）磷脂酶D1蛋白过表达。在31个雌激素受体阳性肿瘤中有6个以及11个雌激素受体阴性肿瘤中有4个磷脂酶D1过表达。在4个显示高CK5/17表达的肿瘤中有3个磷脂酶D1过表达。5个PLD1阳性肿瘤磷酸化 - Akt表达为阴性，但磷酸化 - 哺乳动物雷帕霉素靶蛋白（mTOR）表达为阳性。另外5个PLD1阳性乳腺肿瘤磷酸化 - Akt表达为阳性；然而，这些病例中只有2个磷酸化 - mTOR为阳性。在这项研究中，我们报道在一部分磷酸化 - Akt阴性的乳腺癌中PLD1和磷酸化 - mTOR共表达。
We have used a novel variant of the human oestrogen receptor (ER)-positive MCF-7 cell line, TMX2-28, as a model to study breast cancer. TMX2-28 cells show no detectable levels of mRNA or protein expression for the ER and express basal cytokeratins (CKs) 5, 14, and 17. cDNA microarray comparison between TMX2-28 and its parent cell line, MCF-7, identified 1402 differentially expressed transcripts, one of which was, phospholipase D1 (PLD1). Using real-time RT–PCR, we confirmed that PLD1 mRNA levels are 10-fold higher in TMX2-28 cells than in MCF-7 cells. We next examined PLD1 expression in human breast carcinomas. Phospholipase D1 mRNA levels were higher in breast tumours that expressed high-mRNA levels of basal CKs 5 and/or 17, but PLD1 mRNA levels were not significantly higher in ER-negative tumours. Phospholipase D1 protein was overexpressed in 10 of 42 (24%) breast tumours examined by IHC. Phospholipase D1 was overexpressed in 6 of 31 ER-positive tumours and 4 of 11 ER-negative tumours. Phospholipase D1 was overexpressed in three of the four tumours that showed high CK5/17 expression. Five PLD1-positive tumours were negative for phospho-Akt expression, but positive for phospho-mammalian target of rapamycin (mTOR) expression. The other five PLD1-positive breast tumours showed positive expression for phospho-Akt; however, only two of these cases were positive for phospho-mTOR. In this study, we report that PLD1 and phospho-mTOR are coexpressed in a subset of phospho-Akt-negative breast carcinomas.
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影响因子：
9.2
作者：
Fang, YM;Park, IH;Chen, J
通讯作者：
Chen, J
影响因子：
56.9
作者：
Fang, YM;Vilella-Bach, M;Chen, J
通讯作者：
Chen, J
影响因子：
3.8
作者：
Gozgit, JM;Nestor, KM;Arcaro, KF
通讯作者：
Arcaro, KF
影响因子：
3.8
作者：
Osborne, CK
通讯作者：
Osborne, CK
影响因子：
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作者：
Chen, YH;Zheng, Y;Foster, DA
通讯作者：
Foster, DA