Nutritional influence on fungal colony growth and biomass distribution in response to toxic metals
Nutritional influence on fungal colony growth and biomass distribution in response to toxic metals
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DOI：
10.1016/s0378-1097(01)00399-8
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发表时间：
2001-11-13
影响因子：
2.1
通讯作者：
Ritz, K
中科院分区：
文献类型：
--
作者：
Gadd, GM;Ramsay, L;Ritz, K
这项工作研究营养对真菌菌落生长和生物量分布的影响，以应对有毒金属。在低基质固体培养基中，0.1 mM的镉，铜和锌引起的径向扩展的绿色木霉和少根根霉减少。然而，随着可利用碳源（葡萄糖）量的增加，金属的表观毒性降低。这些金属还影响了真菌菌丝体的总长度和分枝模式。在低营养条件下，绿色木霉表现出整体菌丝长度和分支数量的减少，在一个非常稀疏的分支殖民地。相反，虽然镉也减少了整体菌丝体长度约三分之一的控制长度，分支的数量只略有下降，导致在一个高度分支的殖民地与许多异常的功能。Cu和Cd引起了相似的形态学变化。无根根。一个大规模的菌丝映射技术表明，铜和镉的正常生长中断导致改变生物量分布在殖民地。在无金属低基质培养基上生长时，T。绿球藻的生物量在殖民地内分布均匀，并向周围分布。Cu胁迫下，大部分生物量分布在殖民地外围，而Cd胁迫下，大部分生物量分布在殖民地内部。这些结果意味着，铜和镉的生长和资源分配的这种改变可能会影响成功定位营养物质以及生存，这些金属对生长的真菌有个别和具体的影响。(C)2001年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
This work examines nutritional influence on fungal colony growth and biomass distribution in response to toxic metals. In low-substrate solid medium, 0.1 mM Cd, Cu and Zn caused a decrease in radial expansion of both Trichoderma viride and Rhizopus arrhizus. However, as the amount of available carbon source (glucose) increased, the apparent toxicity of the metals decreased. These metals also affected the overall length of the fungal mycelium and branching patterns. In low-nutrient conditions, T viride showed a decrease in overall mycelial length and number of branches in response to Cu, resulting in an extremely sparsely branched colony. Conversely, although Cd also reduced overall mycelial length to about one-third of the control length, the number of branches decreased only slightly which resulted in a highly branched colony with many aberrant features. Cu and Cd induced similar morphological changes in R. arrhizus. A large-scale mycelial-mapping technique showed that disruption of normal growth by Cu and Cd resulted in altered biomass distribution within the colony. When grown on metal-free low-substrate medium, T. viride showed an even distribution of biomass within the colony with some allocation to the periphery. However, Cu caused most of the biomass to be allocated to the colony periphery, while in the presence of Cd, most biomass was located at the interior of the colony. These results imply that such alterations of growth and resource allocation by Cu and Cd may influence success in locating nutrients as well as survival, and that these metals have individual and specific effects on the growing fungus. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.