Osteogenic differentiation of human mesenchymal stem cells on chargeable polymer-modified surfaces
Osteogenic differentiation of human mesenchymal stem cells on chargeable polymer-modified surfaces
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DOI：
10.1002/jbm.a.31834
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发表时间：
2008-12-15
影响因子：
4.9
通讯作者：
Zhang, Xingdong
中科院分区：
文献类型：
--
作者：
Guo, Likun;Kawazoe, Naoki;Zhang, Xingdong
用正电性聚烯丙基胺(PAAM)和负电性聚丙烯酸(PAAc)修饰的聚苯乙烯细胞培养板和未修饰的聚苯乙烯细胞培养板培养人骨髓间充质干细胞(MSCs)，研究表面静电性质对其成骨分化的影响。所有这些表面都支持细胞的黏附和增殖。然而，细胞在PAAm修饰的表面上的黏附、扩散和增殖比在PAAc修饰的表面和对照表面上更快一些。碱性磷酸酶染色、茜素红S染色和基因表达分析检测成骨分化。加入地塞米松后，骨髓间充质干细胞碱性磷酸酶染色呈阳性，茜素红S染色呈阳性，未加地塞米松培养的细胞未见阳性染色。实时定量聚合酶链式反应的基因表达分析表明，在地塞米松存在下培养的MSCs表达成骨标志物基因，编码碱性磷酸酶、骨钙素、骨涎蛋白、骨桥蛋白和I型胶原。这些结果表明，带正电、带负电和未修饰的表面支持成骨分化，它们的作用需要地塞米松的协同作用。(C)2008年威利期刊公司《生物医学杂志》87A：903-912,2008年
Polystyrene cell-culture plates modified with positively charged polyallylamine (PAAm) and negatively charged poly(acrylic acid) (PAAc) and unmodified plate were used for the culture of human mesenchymal stem cells (MSCs) to study the effect of surface electrostatic properties on their osteogenic differentiation. All of these surfaces supported cell adhesion and proliferation. However, the cells adhered, spread, and proliferated somewhat more quickly on the PAAm-modified surface than they did on the PAAc-modified and control surfaces. Osteogenic differentiation was examined by alkaline phosphatase (ALP) staining, alizarin red S staining, and gene-expression analysis. The MSCs cultured on the three kinds of surfaces in the presence of dexamethasone were positively stained with ALP and alizarin red S staining, while the cells cultured without dexamethasone were not positively stained. Gene-expression analyses using real-time PCR indicated that MSCs cultured on these surfaces in the presence of dexamethasone expressed osteogenic marker genes, encoding ALP, osteocalcin, bone sialoprotein, osteopontin, and type I collagen. These results indicate that the positively charged, negatively charged, and unmodified surfaces supported osteogenic differentiation, and that their effect required the synergistic effect of dexamethasone. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 87A: 903-912, 2008